Western Blot (WB)
(Western blot analysis of PTK9L (arrow) using rabbit polyclonal PTK9L Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PTK9L gene.)
Rabbit anti-Human, mouse PTK9L Polyclonal Antibody | anti-TWF2 antibody
PTK9L Antibody (N-term)
Western Blot (WB)
(Western blot analysis of PTK9L (arrow) using rabbit polyclonal PTK9L Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PTK9L gene.)
Western Blot (WB)
(Western blot analysis of PTK9L (arrow) using rabbit polyclonal PTK9L Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PTK9L gene.)
Western Blot (WB)
(PTK9L Antibody (N-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the PTK9L antibody detected the PTK9L protein (arrow).)
Western Blot (WB)
(PTK9L Antibody (N-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the PTK9L antibody detected the PTK9L protein (arrow).)
Immunohistochemistry (IHC)
(PTK9L Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTK9L Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PTK9L Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTK9L Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(PTK9L Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(PTK9L Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
interaction with the catalytic domain of protein kinase C-zeta. The
encoded protein contains an actin-binding site and an ATP-binding
site. It is most closely related to twinfilin (PTK9), a conserved
actin monomer-binding protein.
Han, S., et al. Hum. Immunol. 71(7):727-730(2010)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 18(5):1651-1658(2009)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein. [provided by RefSeq, Jul 2008]
Uniprot Description
A6r: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. May play a role in regulating the mature length of the middle and short rows of stereocilia. Belongs to the actin-binding proteins ADF family. Twinfilin subfamily.
Protein type: Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 3p21.1
Cellular Component: stereocilium; cytoskeleton; growth cone; myofibril; lamellipodium; perinuclear region of cytoplasm; cytoplasm; filopodium
Molecular Function: actin monomer binding; phosphatidylinositol-4,5-bisphosphate binding; protein kinase C binding; ATP binding
Biological Process: cell projection organization and biogenesis; sequestering of actin monomers; negative regulation of actin filament polymerization; positive regulation of axon extension; regulation of actin cytoskeleton organization and biogenesis; regulation of microvillus length; barbed-end actin filament capping
Research Articles on TWF2
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Product Notes
The TWF2 twf2 (Catalog #AAA9212213) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 28-57. The PTK9L Antibody (N-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PTK9L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the TWF2 twf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PTK9L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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