Rabbit anti-Human, Mouse PTK9L Polyclonal Antibody | anti-PTK9L antibody

PTK9L, NT (TWF2, PTK9L, Twinfilin-2, A6-related protein, Protein tyrosine kinase 9-like, Twinfilin-1-like protein) (FITC)

Cat. Num. AAA6338835

• Gene Names: TWF2; A6r; A6RP; PTK9L; MSTP011
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PTK9L; Polyclonal Antibody; NT (TWF2; Twinfilin-2; A6-related protein; Protein tyrosine kinase 9-like; Twinfilin-1-like protein) (FITC); TWF2; Twinfilin-1-like protein; anti-PTK9L antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PTK9L antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PTK9L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-65 amino acids from the N-terminal region of human PTK9L.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PTK9L (arrow) using rabbit polyclonal PTK9L Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PTK9L gene.)

Western Blot (WB)

(PTK9L Antibody (N-term) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the PTK9L antibody detected the PTK9L protein (arrow).)

Immunohistochemistry (IHC)

(PTK9L Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PTK9L Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(PTK9L Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-PTK9L antibody

The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein.

Product Categories/Family for anti-PTK9L antibody

Antibodies; Protein Kinases

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Han, S., et al. Hum. Immunol. 71(7):727-730(2010). Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 18(5):1651-1658(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 6005846
• NCBI GeneID: 11344
• NCBI Accession #: NP_009215.1
• NCBI GenBank Nucleotide #: NM_007284.3
• UniProt Accession #: Q6IBS0; Q9Y3F5
• Molecular Weight: 39,548 Da
• NCBI Official Full Name: twinfilin-2
• NCBI Official Synonym Full Names: twinfilin actin binding protein 2
• NCBI Official Symbol: TWF2
• NCBI Official Synonym Symbols: A6r; A6RP; PTK9L; MSTP011
• NCBI Protein Information: twinfilin-2; A6-related protein; PTK9L protein tyrosine kinase 9-like (A6-related protein); protein tyrosine kinase 9-like (A6-related protein); twinfilin actin-binding protein 2; twinfilin, actin-binding protein, homolog 2; twinfilin-1-like protein
• UniProt Protein Name: Twinfilin-2
• UniProt Gene Name: TWF2
• UniProt Synonym Gene Names: PTK9L
• UniProt Entry Name: TWF2_HUMAN

NCBI Description

The protein encoded by this gene was identified by its interaction with the catalytic domain of protein kinase C-zeta. The encoded protein contains an actin-binding site and an ATP-binding site. It is most closely related to twinfilin (PTK9), a conserved actin monomer-binding protein. [provided by RefSeq, Jul 2008]