Western Blot (WB)
(All lanes : Anti-NDC80 Antibody (C-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: U-2OS whole cell lysateLane 4: K562 whole cell lysateLane 5: 293T/17 whole cell lysateLane 6: Jurkat whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 74 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human NDC80 Polyclonal Antibody | anti-NDC80 antibody
NDC80 Antibody (C-Term)
Western Blot (WB)
(All lanes : Anti-NDC80 Antibody (C-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: U-2OS whole cell lysateLane 4: K562 whole cell lysateLane 5: 293T/17 whole cell lysateLane 6: Jurkat whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 74 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-NDC80 Antibody (C-Term) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: MCF-7 whole cell lysateLane 3: U-2OS whole cell lysateLane 4: K562 whole cell lysateLane 5: 293T/17 whole cell lysateLane 6: Jurkat whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 74 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]
Uniprot Description
HEC1: plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores.
Protein type: Cell cycle regulation
Chromosomal Location of Human Ortholog: 18p11.32
Cellular Component: kinetochore; membrane; nucleus; cytosol; chromosome, pericentric region
Molecular Function: identical protein binding; protein binding; structural constituent of cytoskeleton
Biological Process: mitosis; mitotic spindle organization and biogenesis; establishment of mitotic spindle orientation; mitotic sister chromatid segregation; attachment of spindle microtubules to kinetochore during mitosis; cell division; small GTPase mediated signal transduction; mitotic cell cycle; attachment of spindle microtubules to kinetochore; chromosome segregation
Research Articles on NDC80
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Product Notes
The NDC80 ndc80 (Catalog #AAA9216438) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDC80 Antibody (C-Term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDC80 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:2000. Researchers should empirically determine the suitability of the NDC80 ndc80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDC80, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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