Rabbit PSMD6 Polyclonal Antibody | anti-PSMD6 antibody

PSMD6 Polyclonal Antibody

Cat. Num. AAA3006060

• Gene Names: PSMD6; S10; Rpn7; p42A; p44S10; SGA-113M
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PSMD6; Polyclonal Antibody; PSMD6 Polyclonal Antibody; 26S proteasome non-ATPase regulatory subunit 6; 26S proteasome regulatory subunit RPN7; 26S proteasome regulatory subunit S10; Breast cancer-associated protein SGA-113M; Phosphonoformate immuno-associated protein 4; Proteasome regulatory particle subunit p44S10; p42A; KIAA0107; PFAAP4; anti-PSMD6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PSMD6 polyclonal antibody detects endogenous levels of PSMD6 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 442

• Applicable Applications for anti-PSMD6 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 252-297 of Human PSMD6.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PSMD6 polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:sp2/0 cell lysateLane3:PC12 cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PSMD6 pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-PSMD6 antibody

In eukaryotic cells, selective breakdown of cellular proteins is ensured by two distinct pathways, ubiquitination and degradation by the 26S proteasome. At specific stages of development, embryo- and tissue-specific components of the 26S proteasome are formed by developmentally regulated alternative splicing, including Rpn10a through Rpn10e (also designated pUb-R2 through pUb-R5). The pUb-R2 subunit, originally identified as S5a, is ubiquitously expressed and may perform proteolysis constitutively in a wide variety of cells. p44S10 is a highly conserved proteasome regulatory subunit that is expressed in heart, liver, skeletal muscle and pancreas. In addition to normal tissue expression, p44S10 is also expressed in several melanoma cell lines, such as MCF-7, 451Lu and WM164. Since forced expression of p44S10 in radial growth phase melanoma cells results in an increase in cellular proliferation, p44S10 may represent a potential link between regulation of proteasome activity and tumor cell proliferation in vivo.

NCBI and Uniprot Product Information

• NCBI GI #: 422398832
• NCBI GeneID: 9861
• NCBI Accession #: NP_001258708.1
• NCBI GenBank Nucleotide #: NP_001258708.1
• UniProt Accession #: Q15008
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 6 isoform 1
• NCBI Official Synonym Full Names: proteasome 26S subunit, non-ATPase 6
• NCBI Official Symbol: PSMD6
• NCBI Official Synonym Symbols: S10; Rpn7; p42A; p44S10; SGA-113M
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 6
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 6
• UniProt Gene Name: PSMD6
• UniProt Synonym Gene Names: KIAA0107; PFAAP4
• UniProt Entry Name: PSMD6_HUMAN

NCBI Description

This gene encodes a member of the protease subunit S10 family. The encoded protein is a subunit of the 26S proteasome which colocalizes with DNA damage foci and is involved in the ATP-dependent degradation of ubiquinated proteins. Alternative splicing results in multiple transcript variants [provided by RefSeq, Nov 2012]

Uniprot Description

PSMD6: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the proteasome subunit S10 family.

Chromosomal Location of Human Ortholog: 3p14.1

Cellular Component: nucleoplasm; proteasome complex; proteasome regulatory particle; cytosol

Molecular Function: ATPase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; proteolysis; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle