Rabbit anti-Human PSMD12 Polyclonal Antibody | anti-PSMD12 antibody

PSMD12, ID (PSMD12, 26S proteasome non-ATPase regulatory subunit 12, 26S proteasome regulatory subunit RPN5, 26S proteasome regulatory subunit p55) (Azide free) (HRP)

Cat. Num. AAA6338253

• Gene Names: PSMD12; p55; Rpn5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PSMD12; Polyclonal Antibody; ID (PSMD12; 26S proteasome non-ATPase regulatory subunit 12; 26S proteasome regulatory subunit RPN5; 26S proteasome regulatory subunit p55) (Azide free) (HRP); 26S proteasome regulatory subunit p55; anti-PSMD12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PSMD12 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PSMD12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 276-304 amino acids from the Central region of human PSMD12.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PSMD12 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the PSMD12 antibody detected the PSMD12 protein (arrow).)

Related Product Information for anti-PSMD12 antibody

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3.

Product Categories/Family for anti-PSMD12 antibody

Antibodies; Proteasomes

References

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Gandhi, T.K., et al. Nat. Genet. 38(3):285-293(2006). Listovsky, T., et al. EMBO J. 23(7):1619-1626(2004). Bouwmeester, T., et al. Nat. Cell Biol. 6(2):97-105(2004). Conticello, S.G., et al. Curr. Biol. 13(22):2009-2013(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4506221
• NCBI GeneID: 5718
• NCBI Accession #: NP_002807.1
• NCBI GenBank Nucleotide #: NM_002816.3
• UniProt Accession #: O00232; Q53HA2; Q6P053; A6NP15
• Molecular Weight: 52,904 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 12 isoform 1
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 12
• NCBI Official Symbol: PSMD12
• NCBI Official Synonym Symbols: p55; Rpn5
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 12; 26S proteasome regulatory subunit p55; 26S proteasome regulatory subunit RPN5
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 12
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD12
• UniProt Entry Name: PSD12_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMD12: Acts as a regulatory subunit of the 26S proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the proteasome subunit p55 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: proteasome complex; nucleoplasm; membrane; proteasome regulatory particle; cytosol

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis