Rabbit anti-Human, Mouse PSMB3 Polyclonal Antibody | anti-PSMB3 antibody

PSMB3 Antibody (Center)

Cat. Num. AAA9232187

• Gene Names: PSMB3; HC10-II
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Synonyms: PSMB3; Polyclonal Antibody; PSMB3 Antibody (Center); Proteasome subunit beta type-3; Proteasome chain 13; Proteasome component C10-II; Proteasome theta chain; anti-PSMB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This PSMB3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 150-185 amino acids from the Central region of human PSMB3.
• Sequence Length: 205

• Applicable Applications for anti-PSMB3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:2000
• Antigen Source: Human
• Function: Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP- dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin- independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
• Cellular Location: Cytoplasm. Nucleus
• Antigen Region: 150-185
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-PSMB3 Antibody (Center) at 1:2000 dilutionLane 1: 293T/17 whole cell lysateLane 2: Hela whole cell lysateLane 3: Jurkat whole cell lysateLane 4: K562 whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-PSMB3 antibody

Description: Purified Rabbit Polyclonal Antibody (Pab)

Background: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Product Categories/Family for anti-PSMB3 antibody

Cell Biology

References

Nothwang H.G.,et al.Biochim. Biophys. Acta 1219:361-368(1994). Bienvenut W.V.,et al.Submitted (JUL-2008) to UniProtKB. Lubec G.,et al.Submitted (MAR-2007) to UniProtKB. Rasmussen H.H.,et al.Electrophoresis 13:960-969(1992). Kristensen P.,et al.Biochem. Biophys. Res. Commun. 205:1785-1789(1994).

NCBI and Uniprot Product Information

• NCBI GI #: 22538465
• NCBI GeneID: 5691
• NCBI Accession #: NP_002786.2
• NCBI GenBank Nucleotide #: NP_002786.2
• UniProt Accession #: P49720
• Molecular Weight: Calculated MW: H=23, M=23, R=23 KDa
• NCBI Official Full Name: proteasome subunit beta type-3
• NCBI Official Synonym Full Names: proteasome 20S subunit beta 3
• NCBI Official Symbol: PSMB3
• NCBI Official Synonym Symbols: HC10-II
• NCBI Protein Information: proteasome subunit beta type-3
• UniProt Protein Name: Proteasome subunit beta type-3
• Protein Family: Proteasome
• UniProt Gene Name: PSMB3
• UniProt Entry Name: PSB3_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The 26 S proteasome may be involved in trinucleotide repeat expansion, a phenomenon which is associated with many hereditary neurological diseases. Pseudogenes have been identified on chromosomes 2 and 12. Alternative splicing results in multiple transcript variants [provided by RefSeq, Sep 2013]

Uniprot Description

PSMB3: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Belongs to the peptidase T1B family.

Protein type: Protease; EC 3.4.25.1; Cell cycle regulation; Proteasome complex

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; Golgi apparatus; mitochondrion; cytoplasm; cytosol; nucleus; actin cytoskeleton

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle