Immunohistochemistry (IHC)
(Staining PSMB7 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Rabbit PSMB7 Polyclonal Antibody | anti-PSMB7 antibody
PSMB7 Antibody (Center)
Predicted: Bovine, Rat
Predicted: Bovine, Rat
WB: 1:8000
Immunohistochemistry (IHC)
(Staining PSMB7 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Staining PSMB7 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
Western Blot (WB)
(All lanes : Anti-PSMB7 Antibody (Center) at 1:8000 dilutionLane 1: A375 whole cell lysateLane 2: Hela whole cell lysateLane 3: HepG2 whole cell lysateLane 4: Jurkat whole cell lysateLane 5: MCF-7 whole cell lysateLane 6: NIH/3T3 whole cell lysateLane 7: Raji whole cell lysate Lysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-PSMB7 Antibody (Center) at 1:8000 dilutionLane 1: A375 whole cell lysateLane 2: Hela whole cell lysateLane 3: HepG2 whole cell lysateLane 4: Jurkat whole cell lysateLane 5: MCF-7 whole cell lysateLane 6: NIH/3T3 whole cell lysateLane 7: Raji whole cell lysate Lysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Background: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the trypsin-like activity.
NCBI and Uniprot Product Information
NCBI Description
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of this gene is located on the long arm of chromosome 14. [provided by RefSeq, Jul 2012]
Uniprot Description
PSMB7: a proteasomal protein of the T1B peptidase family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. Not present in the immunoproteasome and is replaced by catalytic subunit 2i (proteasome beta 10 subunit).
Protein type: Proteasome complex; EC 3.4.25.1; Protease
Chromosomal Location of Human Ortholog: 9q34.11-q34.12
Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; cytoplasm; nucleus; cytosol
Molecular Function: threonine endopeptidase activity; protein binding
Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis
Research Articles on PSMB7
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Product Notes
The PSMB7 psmb7 (Catalog #AAA9232170) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PSMB7 Antibody (Center) reacts with Human, Mouse Predicted: Bovine, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PSMB7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). IHC: 1:25 WB: 1:8000. Researchers should empirically determine the suitability of the PSMB7 psmb7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PSMB7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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