Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Anterior gradient protein 2 homolog (AGR2) Standard Curve.)
Human Anterior Gradient Protein 2 Homolog (AGR2) ELISA Kit | AGR2 elisa kit
Human Anterior Gradient Protein 2 Homolog (AGR2) ELISA Kit
Gene Names
AGR2; AG2; AG-2; HPC8; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-116
Reactivity
Human
Synonyms
Anterior Gradient Protein 2 Homolog (AGR2); Human Anterior Gradient Protein 2 Homolog (AGR2) ELISA Kit; AGR2; AG2; GOB-4; HAG-2; PDIA17; XAG-2; anterior gradient 2 homolog; protein disulfide isomerase family A; member 17; secreted cement gland homolog; AGR2 elisa kit
Reactivity
Human
Specificity
Human Anterior gradient protein 2 homolog (AGR2) ELISA Kit has high sensitivity and excellent specificity for detection of Human AGR2. No significant cross-reactivity or interference between Human AGR2 and analogues was observed.
Sequence Length
175
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Anterior gradient protein 2 homolog (AGR2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Anterior gradient protein 2 homolog (AGR2) Standard Curve.)
Related Product Information for AGR2 elisa kit
Description: This Human Anterior gradient protein 2 homolog (AGR2) ELISA Kit employs a two-site sandwich ELISA to quantitate AGR2 in samples. An antibody specific for AGR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Anterior gradient homolog 2 (AGR2) is a human protein originally discovered in Xenopus laevis, encoded for by the gene of the same name. In Xenopus AGR2 plays a role in cement gland differentiation, however in human cancer cell lines high levels of AGR2 correlate with downregulation of the p53 response and AGR2 levels are elevated in the preneoplastic tissue Barrett's oesophagus. The deduced 175-amino acid soluble AGR2 protein, which is 91% identical to the mouse protein and 47% identical to the frog protein, contains a signal peptide. Northern blot analysis revealed strongest expression of 0.9- and 1.6-kb AGR2 transcripts in lung and in all ER-positive breast carcinoma lines tested; weaker expression was detected in pancreas.
Background: Anterior gradient homolog 2 (AGR2) is a human protein originally discovered in Xenopus laevis, encoded for by the gene of the same name. In Xenopus AGR2 plays a role in cement gland differentiation, however in human cancer cell lines high levels of AGR2 correlate with downregulation of the p53 response and AGR2 levels are elevated in the preneoplastic tissue Barrett's oesophagus. The deduced 175-amino acid soluble AGR2 protein, which is 91% identical to the mouse protein and 47% identical to the frog protein, contains a signal peptide. Northern blot analysis revealed strongest expression of 0.9- and 1.6-kb AGR2 transcripts in lung and in all ER-positive breast carcinoma lines tested; weaker expression was detected in pancreas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
anterior gradient protein 2 homolog
NCBI Official Synonym Full Names
anterior gradient 2, protein disulphide isomerase family member
NCBI Official Symbol
AGR2
NCBI Official Synonym Symbols
AG2; AG-2; HPC8; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-116
NCBI Protein Information
anterior gradient protein 2 homolog
UniProt Protein Name
Anterior gradient protein 2 homolog
Protein Family
UniProt Gene Name
AGR2
UniProt Synonym Gene Names
AG2; AG-2; hAG-2
UniProt Entry Name
AGR2_HUMAN
NCBI Description
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, a catalytically active thioredoxin domain, and a C-terminal ER-retention sequence. This protein plays a role in cell migration, cellular transformation and metastasis and is as a p53 inhibitor. As an ER-localized molecular chaperone, it plays a role in the folding, trafficking, and assembly of cysteine-rich transmembrane receptors and the cysteine-rich intestinal gylcoprotein mucin. This gene has been implicated in inflammatory bowel disease and cancer progression. [provided by RefSeq, Mar 2017]
Research Articles on AGR2
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Product Notes
The Human AGR2 agr2 (Catalog #AAA9714630) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714630 ELISA Kit recognizes Human AGR2. It is sometimes possible for the material contained within the vial of "Anterior Gradient Protein 2 Homolog (AGR2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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