Western Blot (WB)
(Western blot analysis of extracts from CHO-K1 cells, untransfected (A) or transfected with different DYKDDDDK-fusion proteins (B, C), using Myc-Tag (9E10) Mouse mAb (HRP Conjugate) Mouse mAb (1:1000 diluted).)
Mouse Myc-Tag Monoclonal Antibody | anti-Myc-Tag antibody
Myc-Tag (9E10) Mouse mAb (HRP Conjugate)
Applications
Western Blot, Immunoprecipitation, ELISA, Immunohistochemistry
Purity
Affinity Purified
Synonyms
Myc-Tag; Monoclonal Antibody; Myc-Tag (9E10) Mouse mAb (HRP Conjugate); anti-Myc-Tag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgGI
Clone Number
9E10
Purity/Purification
Affinity Purified
Form/Format
In PBS(pH 7.4) containing with 1mg/ml BSA 0.03% Proclin300 and 50% glycerol.
Sequence Length
11
Applicable Applications for anti-Myc-Tag antibody
Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
ELISA: 1:100-1:500
WB: 1:10000-1:50000
WB: 1:10000-1:50000
Immunogen
Synthetic peptide corresponding to Human c-Myc aa 400 to the C-terminus (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
Preparation and Storage
Store at-20 degree C.
Avoid freeze/thaw cycle.
Avoid freeze/thaw cycle.
Western Blot (WB)
(Western blot analysis of extracts from CHO-K1 cells, untransfected (A) or transfected with different DYKDDDDK-fusion proteins (B, C), using Myc-Tag (9E10) Mouse mAb (HRP Conjugate) Mouse mAb (1:1000 diluted).)
Western Blot (WB)
(Western blot analysis of extracts from CHO-K1 cells, untransfected (A) or transfected with different DYKDDDDK-fusion proteins (B, C), using Myc-Tag (9E10) Mouse mAb (HRP Conjugate) Mouse mAb (1:1000 diluted).)
Related Product Information for anti-Myc-Tag antibody
Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
MYC-tag, partial
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Product Notes
The Myc-Tag (Catalog #AAA477426) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Myc-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), ELISA (EIA), Immunohistochemistry (IHC). ELISA: 1:100-1:500 WB: 1:10000-1:50000. Researchers should empirically determine the suitability of the Myc-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Myc-Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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