Goat ATP2A2/SERCA2 Polyclonal Antibody | anti-ATP2A2/SERCA2 antibody

Anti-ATP2A2/SERCA2 Antibody (aa658-671)

Cat. Num. AAA2400938

• Gene Names: ATP2A2; DD; DAR; ATP2B; SERCA2
• Reactivity: Human; Predicted: Rabbit, Mouse, Rat, Monkey
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: ATP2A2/SERCA2; Polyclonal Antibody; Anti-ATP2A2/SERCA2 Antibody (aa658-671); Goat Polyclonal to Human ATP2A2/SERCA2; ATP2A2; ATP2B; Calcium pump 2; DAR; SR Ca(2+)-ATPase 2; Cardiac Ca2+ ATPase; DD; SERCA2; anti-ATP2A2/SERCA2 antibody

• Host: Goat
• Reactivity: Human; Predicted: Rabbit, Mouse, Rat, Monkey
• Clonality: Polyclonal
• Specificity: Human ATP2A2/SERCA2. This antibody is expected to recognize both reported isoforms (NP_001672.1; NP_733765.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Sequence Length: 997

• Applicable Applications for anti-ATP2A2/SERCA2 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: ELISA: 1:128000; IHC-P: 5 ug/ml; WB: 0.1-0.3 ug/ml
• Immunogen: Synthetic peptide DELNPSAQRDACLN from an internal region of human ATP2A2/SERCA2 (NP_001672.1; NP_733765.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Ferret, Panda, Rabbit (100%); Mouse, Rat, Horse, Pig, Guinea pig (93%); Bovine, Elephant, Platypus, Lizard, Xenopus (86%).
• Conjugation: Unconjugated
• Epitope: aa658-671
• Preparation and Storage: Store at -20 degree C.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-ATP2A2/SERCA2 antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(ATP2A2 antibody (0.1 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Heart lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-ATP2A2/SERCA2 antibody

ATP2a2 (Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 ATPase), also known as SERCA2 (Sarcoplasmic reticulum Ca(2+)-ATPase 2), belongs to the large family of P-type cation pumps that couple ATP hydrolysis withcation transport across membranes. the ATP2a2 pump maintains low cytosolic Ca(2+) concentrations by actively transporting Ca(2+) from the cytosol into the sarco/endoplasmic reticulum lumen.

Product Categories/Family for anti-ATP2A2/SERCA2 antibody

Transporter; ATPase - P type; type IIA

NCBI and Uniprot Product Information

• NCBI GI #: 4502285
• NCBI GeneID: 488
• NCBI Accession #: NP_001672.1
• NCBI GenBank Nucleotide #: NM_001681.3
• UniProt Accession #: P16615; P16614; Q86VJ2; A6NDN7; B4DF05
• Molecular Weight: 109,734 Da
• NCBI Official Full Name: sarcoplasmic/endoplasmic reticulum calcium ATPase 2 isoform a
• NCBI Official Synonym Full Names: ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2
• NCBI Official Symbol: ATP2A2
• NCBI Official Synonym Symbols: DD; DAR; ATP2B; SERCA2
• NCBI Protein Information: sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• UniProt Protein Name: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2
• UniProt Gene Name: ATP2A2
• UniProt Synonym Gene Names: ATP2B; SERCA2; SR Ca(2+)-ATPase 2

NCBI Description

This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Isoform 2 is involved in the regulation of the contraction/relaxation cycle (PubMed:16402920). Acts as a regulator of TNFSF11-mediated Ca2+ signaling pathways via its interaction with TMEM64 which is critical for the TNFSF11-induced CREB1 activation and mitochondrial ROS generation necessary for proper osteoclast generation. Association between TMEM64 and SERCA2 in the ER leads to cytosolic Ca (2+) spiking for activation of NFATC1 and production of mitochondrial ROS, thereby triggering Ca (2+) signaling cascades that promote osteoclast differentiation and activation ().