Rabbit PSAT1 Polyclonal Antibody | anti-PSAT1 antibody

Anti-PSAT1 Antibody

Cat. Num. AAA178842

• Gene Names: PSAT1; PSA; EPIP; NLS2; PSAT; PSATD
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen affinity purified.
• Synonyms: PSAT1; Polyclonal Antibody; Anti-PSAT1 Antibody; EPIP; MGC1460; NLS2; PSA; PSAT; Psat1; PSATD; Q9Y617; Phosphoserine aminotransferase; phosphoserine aminotransferase 1; anti-PSAT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 324

• Applicable Applications for anti-PSAT1 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot: 0.1-0.5ug/ml; Immunohistochemistry(IHC) Paraffin: 0.5-1ug/ml
• Notes: Tested Species: In-house tested species with positive results.; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.; Other applications have not been tested.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Immunogen: E. coli-derived human PSAT1 recombinant protein (Position: Q276-L370). Human PSAT1 shares 90.5% amino acid (aa) sequence identity with mouse PSAT1.
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of PSAT1 expression in rat pancreas extract (lane 1) and HELA whole cell lysates (lane 2). PSAT1 at 40KD was detected using rabbit anti- PSAT1 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Immunohistochemistry (IHC)

(PSAT1 was detected in paraffin-embedded sections of mouse liver tissues using rabbit anti- PSAT1 Antigen Affinity purified polyclonal antibody at 1 ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(PSAT1 was detected in paraffin-embedded sections of rat liver tissues using rabbit anti- PSAT1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Immunohistochemistry (IHC)

(PSAT1 was detected in paraffin-embedded sections of human mammary cancer tissues using rabbit anti- PSAT1 Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method. )

Related Product Information for anti-PSAT1 antibody

Rabbit IgG polyclonal antibody for Phosphoserine aminotransferase(PSAT1) detection.
Background: Phosphoserine aminotransferase (PSA), also known as phosphohydroxythreonine aminotransferase (PSAT), is an enzyme that in humans is encoded by the PSAT1 gene. This gene encodes a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is a phosphoserine aminotransferase and decreased expression may be associated with schizophrenia. Mutations in this gene are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants.

References

1. Hart CE, Race V, Achouri Y, Wiame E, Sharrard M, Olpin SE, Watkinson J, Bonham JR, Jaeken J, Matthijs G, Van Schaftingen E (2007). "Phosphoserine aminotransferase deficiency: a novel disorder of the serine biosynthesis pathway". Am. J. Hum. Genet. 80 (5): 931-7.
2. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, Iyer V, Mujica AO, Thomas M, Harrow J, Cox T, Jackson D, Severin J, Biggs P, Fu J, Nefedov M, de Jong PJ, Stewart AF, Bradley A (2011). "A conditional knockout resource for the genome-wide study of mouse gene function". Nature 474 (7351): 337-342.

NCBI and Uniprot Product Information

• NCBI GI #: 10863955
• NCBI GeneID: 29968
• NCBI Accession #: NP_066977.1
• NCBI GenBank Nucleotide #: NM_021154.4
• UniProt Accession #: Q9Y617; Q5T7G5; Q5T7G6; Q96AW2; Q9BQ12
• Molecular Weight: 35,189 Da
• NCBI Official Full Name: phosphoserine aminotransferase isoform 2
• NCBI Official Synonym Full Names: phosphoserine aminotransferase 1
• NCBI Official Symbol: PSAT1
• NCBI Official Synonym Symbols: PSA; EPIP; NLS2; PSAT; PSATD
• NCBI Protein Information: phosphoserine aminotransferase
• UniProt Protein Name: Phosphoserine aminotransferase
• Protein Family: Phosphoserine aminotransferase
• UniProt Gene Name: PSAT1
• UniProt Synonym Gene Names: PSA; PSAT

NCBI Description

This gene encodes a member of the class-V pyridoxal-phosphate-dependent aminotransferase family. The encoded protein is a phosphoserine aminotransferase and decreased expression may be associated with schizophrenia. Mutations in this gene are also associated with phosphoserine aminotransferase deficiency. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, and 8. [provided by RefSeq, Jul 2013]

Uniprot Description

PSAT1: Catalyzes the reversible conversion of 3- phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4- phosphonooxybutanoate to phosphohydroxythreonine. Defects in PSAT1 are the cause of phosphoserine aminotransferase deficiency (PSATD). PSATD is characterized biochemically by low plasma and cerebrospinal fluid concentrations of serine and glycine and clinically by intractable seizures, acquired microcephaly, hypertonia, and psychomotor retardation. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Cofactor and Vitamin Metabolism - vitamin B6; EC 2.6.1.52; Transferase

Chromosomal Location of Human Ortholog: 9q21.2

Cellular Component: cytoplasm; cytosol

Molecular Function: phosphoserine transaminase activity

Biological Process: L-serine biosynthetic process

Disease: Neu-laxova Syndrome 2; Phosphoserine Aminotransferase Deficiency