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Typical Testing Data

Rat Gastric inhibitory polypeptide ELISA Kit | Gip elisa kit

Rat Gastric inhibitory polypeptide ELISA Kit

Reactivity
Rat
Synonyms
Gastric inhibitory polypeptide; Rat Gastric inhibitory polypeptide ELISA Kit; Gip/gastric inhibitory polypeptide; Gip elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Sequence Length
153
Samples
Serum, plasma and other biological fluids.
Assay Type
Sandwich ELISA, Double Antibody
Detection Range
0.625-40ng/ml
Sensitivity
0.375ng/ml
Preparation and Storage
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Testing Data

Typical Standard Curve

Typical Standard Curve
Related Product Information for Gip elisa kit
Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Gip antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Gip antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Gip amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Gip can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17,108 Da
NCBI Official Full Name
gastric inhibitory polypeptide preproprotein
NCBI Official Synonym Full Names
gastric inhibitory polypeptide
NCBI Official Symbol
GIP
NCBI Protein Information
gastric inhibitory polypeptide
UniProt Protein Name
Gastric inhibitory polypeptide
UniProt Gene Name
GIP
UniProt Synonym Gene Names
GIP
UniProt Entry Name
GIP_HUMAN

NCBI Description

This gene encodes an incretin hormone and belongs to the glucagon superfamily. The encoded protein is important in maintaining glucose homeostasis as it is a potent stimulator of insulin secretion from pancreatic beta-cells following food ingestion and nutrient absorption. This gene stimulates insulin secretion via its G protein-coupled receptor activation of adenylyl cyclase and other signal transduction pathways. It is a relatively poor inhibitor of gastric acid secretion. [provided by RefSeq, Jul 2008]

Uniprot Description

GIP: Potent stimulator of insulin secretion and relatively poor inhibitor of gastric acid secretion. Belongs to the glucagon family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21.3-q22

Cellular Component: cell soma; endoplasmic reticulum lumen; extracellular region; extracellular space

Molecular Function: hormone activity; protein binding

Biological Process: adult locomotory behavior; cellular protein metabolic process; endocrine pancreas development; female pregnancy; memory; positive regulation of insulin secretion; regulation of insulin secretion; response to acidity; response to amino acid stimulus; response to axon injury; response to drug; response to glucose stimulus; response to lipid; response to organic cyclic substance; response to peptide hormone stimulus; response to selenium ion; response to starvation; sensory perception of pain; signal transduction

Research Articles on Gip

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Product Notes

The Rat Gip gip (Catalog #AAA764839) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA764839 ELISA Kit recognizes Rat Gip. It is sometimes possible for the material contained within the vial of "Gastric inhibitory polypeptide, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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