Rabbit PRPF3 Polyclonal Antibody | anti-PRPF3 antibody

PRPF3 Polyclonal Antibody

Cat. Num. AAA3011293

• Gene Names: PRPF3; PRP3; RP18; HPRP3; Prp3p; HPRP3P; SNRNP90
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PRPF3; Polyclonal Antibody; PRPF3 Polyclonal Antibody; hPrp3; HPRP3P; Pre mRNA splicing factor 3; Pre-mRNA-splicing factor 3; PRP3 pre-mRNA processing factor 3 homolog (S. cerevisiae); Prp3p; PRPF3_HUMAN; RP18; U4/U6 small nuclear ribonucleoprotein PRP3; U4/U6 snRNP 90 kDa protein; anti-PRPF3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PRPF3 polyclonal antibody detects endogenous levels of PRPF3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 683

• Applicable Applications for anti-PRPF3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200; IP: 1:50-1:100
• Immunogen: Recombinant full length Human PRPF3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PRPF3 polyclonal antibody)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells, using PRPF3 polyclonal antibody)

Related Product Information for anti-PRPF3 antibody

PRPF3 (PRP3 pre-mRNA processing factor 3 homolog), also known as RP18, PRP3, Prp3p, HPRP3 or HPRP3P, is an evolutionarily conserved protein involved in pre-mRNA splicing and functions as a component of the U4/U6.U5 tri-snRNP (small nuclear ribonucleoprotein) complex. Ubiquitously expressed with predominant expression in retina, blood, kidney and liver, PRPF3 localizes to nuclear speckles and is phosphorylated in vitro. PRPF3 directly interacts with PRPF4 and is present in the inactive spliceosome but is not found in the catalytically active spliceosome. Mutations in the gene encoding PRPF3 result in autosomal dominant retinitis pigmentosa type 18 (RP18), which leads to photoreceptor cell degeneration. RP18 patients initially exhibit a loss of their midperipheral visual field as well as night vision blindness. The disease eventually progresses to the loss of far peripheral visual field and finally the loss of central vision. This suggests that PRPF3 is a key player in the pre-mRNA splicing of photoreceptor-specific genes.

NCBI and Uniprot Product Information

• NCBI GI #: 4758556
• NCBI GeneID: 9129
• NCBI Accession #: NP_004689.1
• NCBI GenBank Nucleotide #: NP_004689.1
• UniProt Accession #: O43395
• Molecular Weight: ~ 100kDa
• NCBI Official Full Name: U4/U6 small nuclear ribonucleoprotein Prp3 isoform 1
• NCBI Official Synonym Full Names: pre-mRNA processing factor 3
• NCBI Official Symbol: PRPF3
• NCBI Official Synonym Symbols: PRP3; RP18; HPRP3; Prp3p; HPRP3P; SNRNP90
• NCBI Protein Information: U4/U6 small nuclear ribonucleoprotein Prp3
• UniProt Protein Name: U4/U6 small nuclear ribonucleoprotein Prp3
• Protein Family: U4/U6 small nuclear ribonucleoprotein
• UniProt Gene Name: PRPF3
• UniProt Synonym Gene Names: HPRP3; PRP3; hPrp3
• UniProt Entry Name: PRPF3_HUMAN

NCBI Description

The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18. [provided by RefSeq, Jul 2008]

Uniprot Description

PRPF3: Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex. Defects in PRPF3 are the cause of retinitis pigmentosa type 18 (RP18). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP18 inheritance is autosomal dominant.

Protein type: RNA splicing; Spliceosome; RNA processing

Chromosomal Location of Human Ortholog: 1q21.1

Cellular Component: nucleoplasm; Cajal body; spliceosome; U4/U6 x U5 tri-snRNP complex; cytoplasm; nuclear speck; nucleus

Molecular Function: identical protein binding; protein binding

Biological Process: assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome; RNA splicing, via transesterification reactions; RNA splicing; mRNA processing

Disease: Retinitis Pigmentosa 18