Mouse VDAC1 Monoclonal Antibody | anti-VDAC1 antibody

VDAC1 Monoclonal Antibody

Cat. Num. AAA191348

• Gene Names: VDAC1; PORIN; VDAC-1
• Applications: Immunoblot, Western Blot, Immunofluorescence
• Synonyms: VDAC1; Monoclonal Antibody; VDAC1 Monoclonal Antibody; anti-VDAC1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Specificity: This antibody recognizes human, mouse and rat VDAC1. It does not cross-react with VDAC2 or VDAC3 (based on evaluation in knock-out mice).
• Form/Format: 100ug (1mg/ml) Protein G-purified antibody in PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.
• Sequence Length: 283

• Applicable Applications for anti-VDAC1 antibody: Immunoblot, Immunofluorescence (IF)
• Application Notes: Immunoblot: 1-5ug/ml. A band of ~30kDa is detected.; IF: 10ug/ml
• Immunogen: Fusion protein corresponding to aa 1-283 (full-length) of human VDAC1. This sequence is 98% identical to mouse, 98% identical to rat, and >60% identical to VDAC2 and VCAC3.

Testing Data

(Detection of VDAC1 in rat brain lysates at 5ug/ml.)

Testing Data

(Detection of VDAC1 in neuroblastoma cell line SK-N-BE at 10ug/ml: DAPI (blue) nuclear stain, Texas Red F actin stain, ATTO 488 (green) VDAC1 stain.)

Related Product Information for anti-VDAC1 antibody

VDAC1 (also known as Voltage-dependent anion-selective channel protein 1 and outer mitochondrial membrane protein porin 1) is the outer mitochondrial membrane receptor for hexokinase and BCL2L1. VDAC1 forms a channel through the mitochondrial membrane and is involved in small molecule diffusion, cell volume regulation, and apoptosis. VDAC1 may participate in the formation of the permeability transition pore complex (PTPC) which is responsible for the release of mitochondrial products that trigger apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 4507879
• NCBI GeneID: 7416
• NCBI Accession #: NP_003365.1
• NCBI GenBank Nucleotide #: NM_003374.2
• UniProt Accession #: P21796; Q5FVE7; Q9UIQ5; Q9UPL0; B3KVK4; D3DQ93
• Molecular Weight: 30,773 Da
• NCBI Official Full Name: voltage-dependent anion-selective channel protein 1
• NCBI Official Synonym Full Names: voltage dependent anion channel 1
• NCBI Official Symbol: VDAC1
• NCBI Official Synonym Symbols: PORIN; VDAC-1
• NCBI Protein Information: voltage-dependent anion-selective channel protein 1
• UniProt Protein Name: Voltage-dependent anion-selective channel protein 1
• Protein Family: Voltage-dependent anion-selective channel protein
• UniProt Gene Name: VDAC1
• UniProt Synonym Gene Names: VDAC; VDAC-1; hVDAC1

NCBI Description

This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.[provided by RefSeq, Sep 2010]

Uniprot Description

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:11845315, PubMed:18755977, PubMed:20230784, PubMed:8420959). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (PubMed:15033708, PubMed:25296756).