Rabbit PRPF19 Polyclonal Antibody | anti-PRPF19 antibody

PRPF19 Polyclonal Antibody

Cat. Num. AAA3007462

• Gene Names: PRPF19; PSO4; SNEV; PRP19; UBOX4; hPSO4; NMP200
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: PRPF19; Polyclonal Antibody; PRPF19 Polyclonal Antibody; Pre-mRNA-processing factor 19; Nuclear matrix protein 200; PRP19/PSO4 homolog; hPso4; Senescence evasion factor; anti-PRPF19 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PRPF19 polyclonal antibody detects endogenous levels of PRPF19 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 504

• Applicable Applications for anti-PRPF19 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 165-209 of Human PRPF19
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PRPF19 polyclonal antibody at 1:500 dilutionLane1:PC3 whole cell lysate (40ug) Lane2:HCT116 whole cell lysate (40ug) Lane3:The Kidney lysate of Rat (40ug) Lane4:The Heart lysate of Mouse (40ug) Lane5:The Liver lysate of Mouse (40ug))

Related Product Information for anti-PRPF19 antibody

The spliceosome, the gigantic molecular machine that performs pre-mRNA splicing in eukaryotes, contains over 200 different proteins and 5 RNA molecules (U1, U2, U4, U5 and U6). Pre-mRNA splicing is essential to remove internal non-coding regions of pre-mRNA (introns) and to join the remaining segments (exons) into mRNA before translation. The PRP19-associated complex is required for stable association of U5 and U6 with the spliceosome after U4 is released. Changes within the spliceosome upon binding of the PRP19-associated complex include remodeling of the U6/5' splice site interaction and destabilization of Lsm proteins to allow further interaction of U6 with the intron sequence.

NCBI and Uniprot Product Information

• NCBI GI #: 7657381
• NCBI GeneID: 27339
• NCBI Accession #: NP_055317.1
• NCBI GenBank Nucleotide #: NP_055317.1
• UniProt Accession #: Q9UMS4
• Molecular Weight: ~ 55kDa
• NCBI Official Full Name: pre-mRNA-processing factor 19
• NCBI Official Synonym Full Names: pre-mRNA processing factor 19
• NCBI Official Symbol: PRPF19
• NCBI Official Synonym Symbols: PSO4; SNEV; PRP19; UBOX4; hPSO4; NMP200
• NCBI Protein Information: pre-mRNA-processing factor 19
• UniProt Protein Name: Pre-mRNA-processing factor 19
• UniProt Gene Name: PRPF19
• UniProt Synonym Gene Names: NMP200; PRP19; SNEV; hPso4
• UniProt Entry Name: PRP19_HUMAN

NCBI Description

PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]).[supplied by OMIM, Sep 2008]

Uniprot Description

PRPF19: Plays a role in DNA double-strand break (DSB) repair. Binds double-stranded DNA in a sequence-nonspecific manner. Acts as a structural component of the nuclear framework. May also serve as a support for spliceosome binding and activity. Essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. May have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Belongs to the WD repeat PRP19 family.

Protein type: RNA splicing; Ubiquitin conjugating system; Ligase; EC 6.3.2.-; Spliceosome; Ubiquitin ligase; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 11q12.2

Cellular Component: nucleoplasm; DNA replication factor A complex; membrane; cytoplasm; spindle; nuclear speck; lipid particle; nucleus

Molecular Function: identical protein binding; protein binding; ligase activity

Biological Process: positive regulation of nuclear mRNA splicing, via spliceosome; assembly of spliceosomal tri-snRNP; nuclear mRNA splicing, via spliceosome; proteasomal protein catabolic process; negative regulation of neuron differentiation; protein polyubiquitination; RNA splicing; lipid biosynthetic process; spliceosome assembly; gene expression; generation of catalytic spliceosome for first transesterification step; inner cell mass cell proliferation; double-strand break repair via nonhomologous end joining; positive regulation of astrocyte differentiation