Rabbit anti-Human Proteasome 19S Rpt2/S4 subunit Polyclonal Antibody | anti-PSMC1 antibody

Proteasome 19S Rpt2/S4 subunit (human) polyclonal antibody

Cat. Num. AAA565255

• Gene Names: PSMC1; S4; p56; P26S4
• Reactivity: Human
• Applications: Western Blot
• Purity: Partially purified by salt precipitation.
• Synonyms: Proteasome 19S Rpt2/S4 subunit; Polyclonal Antibody; Proteasome 19S Rpt2/S4 subunit (human) polyclonal antibody; Proteasome 26S subunit ATPase 1; 26S protease regulatory subunit 4; anti-PSMC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human proteasome 19S (Rpt2/S4 subunit).
• Purity/Purification: Partially purified by salt precipitation.
• Form/Format: Liquid. In PBS containing 0.01% sodium azide.
• Sequence Length: 440

• Applicable Applications for anti-PSMC1 antibody: Western Blot (WB)
• Application Notes: Western Blot (1:1,000); Suggested dilutions/conditions may not be available for all applications.
• Immunogen: Synthetic peptide corresponding to aa 368-383 of human proteasome 19S (Rpt2/S4 subunit).
• Preparation and Storage: Long term: -20 degree C; Short term: 4 degree C; Stable for at least 1 year after receipt when stored at -20 degree C. Dilute with PBS, pH 7.2-7.4, containing 1% normal goat serum (if a goat anti-rabbit IgG linker antibody is to be used). Store diluted antibody at +4 degree C, do not freeze and use within 1 month.; Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20 degree C.

Western Blot (WB)

(Figure: Western blot showing detection of Rpt2/S4 19S regulatory subunit. Lane 1: Purified 26S proteasome . Lane 2: HeLa S100 cytosolic fraction . PAb to Proteasome 19S (Rpt2/S4 Subunit) (human) at 1:1'000 dilution.)

Related Product Information for anti-PSMC1 antibody

The 19S regulatory complex consists of a 'base' sub-complex, composed of a hetero-hexameric ring of AAA-ATPases that not only function as the gate-opener to the 20S proteasome but also, in conjunction with two other high-molecular-mass non-ATPase subunits (Rpn2/S1 and Rpn1/S2), as a platform for protein unfolding. The 'lid' sub-complex of the 19S complex, composed of non-ATPase subunits is involved in the binding and deubiquitinylation of proteasome targeted ubiquitin conjugated proteins and assists in the transport of substrates through the 'base' and into the 20S catalytic core.

Product Categories/Family for anti-PSMC1 antibody

Proteolysis & Protein Degradation; Protein Synthesis & Folding

NCBI and Uniprot Product Information

• NCBI GI #: 49065817
• NCBI GeneID: 5700
• UniProt Accession #: P62191; P49014; Q03527; Q6IAW0; Q6NW36; Q96AZ3; B4DR63
• Molecular Weight: 41,167 Da
• NCBI Official Full Name: 26S protease regulatory subunit 4
• NCBI Official Synonym Full Names: proteasome 26S subunit, ATPase 1
• NCBI Official Symbol: PSMC1
• NCBI Official Synonym Symbols: S4; p56; P26S4
• NCBI Protein Information: 26S protease regulatory subunit 4
• UniProt Protein Name: 26S protease regulatory subunit 4
• UniProt Gene Name: PSMC1
• UniProt Synonym Gene Names: P26s4
• UniProt Entry Name: PRS4_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases which have a chaperone-like activity. This subunit and a 20S core alpha subunit interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. This subunit also interacts with the adenovirus E1A protein and this interaction alters the activity of the proteasome. Finally, this subunit interacts with ataxin-7, suggesting a role for the proteasome in the development of spinocerebellar ataxia type 7, a progressive neurodegenerative disorder. [provided by RefSeq, Jul 2008]

Uniprot Description

RPT2: The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. Belongs to the AAA ATPase family.

Protein type: Protease; Proteasome complex

Chromosomal Location of Human Ortholog: 14q32.11

Cellular Component: cytoplasm; cytosol; membrane; nucleoplasm; nucleus; proteasome complex

Molecular Function: ATP binding; ATPase activity; protein binding; TATA-binding protein binding

Biological Process: activation of MAPKK activity; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; apoptosis; axon guidance; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; epidermal growth factor receptor signaling pathway; ER-associated protein catabolic process; fibroblast growth factor receptor signaling pathway; G1/S transition of mitotic cell cycle; gene expression; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; mitotic cell cycle; negative regulation of apoptosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; nerve growth factor receptor signaling pathway; polyamine metabolic process; positive regulation of transcriptional preinitiation complex assembly; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; programmed cell death; protein polyubiquitination; Ras protein signal transduction; regulation of amino acid metabolic process; regulation of apoptosis; regulation of mRNA stability; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; small GTPase mediated signal transduction; stimulatory C-type lectin receptor signaling pathway; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway; vascular endothelial growth factor receptor signaling pathway; viral reproduction