Goat anti-Mouse Prostasin Polyclonal Antibody | anti-PRSS8 antibody
Prostasin (Prss8, CAP1, PROSTASIN, Serine protease 8, Protease, serine, 8)
Gene Names
PRSS8; CAP1; PROSTASIN; PRSS8
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Prostasin; Polyclonal Antibody; Prostasin (Prss8; CAP1; PROSTASIN; Serine protease 8; Protease; serine; 8); Anti -Prostasin (Prss8; anti-PRSS8 antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Prostasin.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Applicable Applications for anti-PRSS8 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA (Capture): 0.2-0.8ug/ml
Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Prostasin. The detection limit for recombinant mouse Prostasin is ~20ng/lane under non-reducing and reducing conditions.
Dilution: ELISA (Capture): 0.2-0.8ug/ml
Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Prostasin. The detection limit for recombinant mouse Prostasin is ~20ng/lane under non-reducing and reducing conditions.
Immunogen
Purified recombinant protein from mouse Prostasin expressed in NSO cells
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-PRSS8 antibody
PRSS8 is a member of the trypsin family of serine proteases. It can be found in seminal fluid and is highly expressed in prostate epithelia. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. The carboxyterminal region contains a GPI anchor sequence, which anchors prostasin to the cell surface. Phospholipase activity is thought to liberate prostasin from the cell, and cleavage of the linker region also releases prostasin. PRSS8 has been proposed as a marker for ovarian cancer, where it is up-regulated, a marker for androgen-refractory prostate cancer, where it is down-regulated, and as an inhibitor of invasiveness in breast cancer.
Product Categories/Family for anti-PRSS8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
36,431 Da
NCBI Official Full Name
prostasin
NCBI Official Synonym Full Names
protease, serine, 8
NCBI Official Symbol
PRSS8
NCBI Official Synonym Symbols
CAP1; PROSTASIN; PRSS8
NCBI Protein Information
prostasin; serine protease 8; channel-activating protease 1
UniProt Protein Name
Prostasin
Protein Family
UniProt Gene Name
PRSS8
UniProt Entry Name
PRSS8_HUMAN
NCBI Description
This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is highly expressed in prostate epithelia and is one of several proteolytic enzymes found in seminal fluid. The proprotein is cleaved to produce a light chain and a heavy chain which are associated by a disulfide bond. It is active on peptide linkages involving the carboxyl group of lysine or arginine. [provided by RefSeq]
Uniprot Description
PRSS8: Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates. Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G). Belongs to the peptidase S1 family.
Protein type: Membrane protein, integral; EC 3.4.21.-; Protease
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: extracellular space; integral to membrane; plasma membrane; extracellular region
Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: proteolysis
Research Articles on PRSS8
1. Hepsin activates prostasin and cleaves the extracellular domain of the epidermal growth factor receptor.
2. Luciferase assay using CYP11B2 promoter revealed that prostasin significantly increased the transcriptional activity of CYP11B2.
3. Prostasin may regulate trophoblast cell proliferation via modulating the EGFR-MAPK signaling pathway.
4. In the in vitro cultured human choriocarcinomal JEG-3 cells, treatment with functional antibody against prostasin led to promotion in cell invasion capability, as well as increase in the production of MMP-2, MMP-26, TIMP-1, and TIMP-4.
5. Loss of prostasin expression in bladder transitional cell carcinomas is associated with epithelial-mesenchymal transition.
6. the mRNA level of PN-1 is significantly elevated in colorectal cancer tissue
7. it is not clear, at the present time, whether endogenous aldosterone regulates prostasin expression or vice versa
8. study presents structures of the extracellular domain of active prostasin in several forms; of particular interest is the S1 subsite loop which opens and closes in response to basic residues or divalent ions, directly binding Ca(+2) cations
9. Suggest that urinary prostasin could be a novel biomarker and/or mechanism for renal pressure natriuresis in normotensive black adolescents.
10. crystallographic analysis of prostasin
2. Luciferase assay using CYP11B2 promoter revealed that prostasin significantly increased the transcriptional activity of CYP11B2.
3. Prostasin may regulate trophoblast cell proliferation via modulating the EGFR-MAPK signaling pathway.
4. In the in vitro cultured human choriocarcinomal JEG-3 cells, treatment with functional antibody against prostasin led to promotion in cell invasion capability, as well as increase in the production of MMP-2, MMP-26, TIMP-1, and TIMP-4.
5. Loss of prostasin expression in bladder transitional cell carcinomas is associated with epithelial-mesenchymal transition.
6. the mRNA level of PN-1 is significantly elevated in colorectal cancer tissue
7. it is not clear, at the present time, whether endogenous aldosterone regulates prostasin expression or vice versa
8. study presents structures of the extracellular domain of active prostasin in several forms; of particular interest is the S1 subsite loop which opens and closes in response to basic residues or divalent ions, directly binding Ca(+2) cations
9. Suggest that urinary prostasin could be a novel biomarker and/or mechanism for renal pressure natriuresis in normotensive black adolescents.
10. crystallographic analysis of prostasin
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Product Notes
The PRSS8 prss8 (Catalog #AAA621678) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Prostasin (Prss8, CAP1, PROSTASIN, Serine protease 8, Protease, serine, 8) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Prostasin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA (Capture): 0.2-0.8ug/ml Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse Prostasin. The detection limit for recombinant mouse Prostasin is ~20ng/lane under non-reducing and reducing conditions. Researchers should empirically determine the suitability of the PRSS8 prss8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prostasin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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