Rabbit anti-Human PROM2 Polyclonal Antibody | anti-PROM2 antibody

PROM2, ID (PROM2, PROML2, Prominin-2, Prominin-like protein 2) (Azide free) (HRP)

Cat. Num. AAA6337571

• Gene Names: PROM2; PROML2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PROM2; Polyclonal Antibody; ID (PROM2; PROML2; Prominin-2; Prominin-like protein 2) (Azide free) (HRP); Prominin-like protein 2; anti-PROM2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-PROM2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PROM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 439-466 amino acids from the Central region of human PROM2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PROM2 Antibody (Center) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the PROM2 antibody detected the PROM2 protein (arrow).)

Related Product Information for anti-PROM2 antibody

This gene encodes a member of the prominin family of pentaspan membrane glycoproteins. The encoded protein localizes to basal epithelial cells and may be involved in the organization of plasma membrane microdomains. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-PROM2 antibody

Antibodies; Proteins

References

Jaszai, J., et al. Histochem. Cell Biol. 133(5):527-539(2010). Yao, J., et al. Oncol. Rep. 22(4):781-787(2009). Jaszai, J., et al. Histochem. Cell Biol. 130(4):749-759(2008). Fargeas, C.A., et al. J. Biol. Chem. 278(10):8586-8596(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 260764013
• NCBI GeneID: 150696
• NCBI Accession #: NP_001159449.1
• NCBI GenBank Nucleotide #: NM_001165977.1
• UniProt Accession #: Q8N271; Q2HIX6; Q8NB84; Q8TAE2; A8K2V1
• Molecular Weight: 91,883 Da
• NCBI Official Full Name: prominin-2
• NCBI Official Synonym Full Names: prominin 2
• NCBI Official Symbol: PROM2
• NCBI Official Synonym Symbols: PROML2
• NCBI Protein Information: prominin-2; hPROML2; prominin-like protein 2; prominin-related protein
• UniProt Protein Name: Prominin-2
• Protein Family: Prominin
• UniProt Gene Name: PROM2
• UniProt Synonym Gene Names: PROML2; PROM-2; hPROML2
• UniProt Entry Name: PROM2_HUMAN

NCBI Description

This gene encodes a member of the prominin family of pentaspan membrane glycoproteins. The encoded protein localizes to basal epithelial cells and may be involved in the organization of plasma membrane microdomains. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

PROM2: a multi-pass membrane protein of the prominin family that binds cholesterol. Localizes to the apical and basolateral membranes of epithelial cells. Present in saliva within small membrane particles. Expressed in kidney, prostate, trachea, esophagus, salivary gland, thyroid gland, mammary gland adrenal gland, placenta, stomach, spinal cord and liver. In submucosal tumor, expressed in spindle-shaped or stellate stromal cells. Expressed in prostate cancer cell lines. Three alternatively spliced human isoforms have been described.

Protein type: Cell surface; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q11.1

Cellular Component: microvillus; cell surface; cell projection; microvillus membrane; integral to plasma membrane; basolateral plasma membrane; apical plasma membrane; cytoplasmic vesicle; lipid raft; cilium

Molecular Function: cholesterol binding

Biological Process: positive regulation of cell projection organization and biogenesis; positive regulation of protein amino acid phosphorylation; negative regulation of pinocytosis