Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prominin-2 (PROM2) Standard Curve.)
Mouse Prominin-2 (PROM2) ELISA Kit | PROM2 elisa kit
Mouse Prominin-2 (PROM2) ELISA Kit
Gene Names
Prom2; Prom-rp; AI507243
Reactivity
Mouse
Synonyms
Prominin-2 (PROM2); Mouse Prominin-2 (PROM2) ELISA Kit; PROM2; MGC138714; PROML2; OTTHUMP00000207357; prominin-like protein 2; prominin-related protein; PROM2 elisa kit
Reactivity
Mouse
Specificity
Mouse Prominin-2 (PROM2) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PROM2. No significant cross-reactivity or interference between Mouse PROM2 and analogues was observed.
Sequence Length
835
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prominin-2 (PROM2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Prominin-2 (PROM2) Standard Curve.)
Related Product Information for PROM2 elisa kit
Description: This Mouse Prominin-2 (PROM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PROM2 in samples. An antibody specific for PROM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PROM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Prominin 2 is a member of the prominin family of pentaspan membrane glycoproteins. The encoded protein localizes to basal epithelial cells and may be involved in the organization of plasma membrane microdomains. Alternative splicing results in multiple transcript variants.Colocalizes with PROM1. Associates with membrane in a cholesterol-dependent manner. Localizes to the apical and basolateral membranes of epithelial cells Present in saliva within small membrane particles (at protein level). Expressed in kidney, prostate, trachea, esophagus, salivary gland, thyroid gland, mammary gland adrenal gland, placenta, stomach, spinal cord and liver. In submucosal tumor, expressed in spindle-shaped or stellate stromal cells. Expressed in prostate cancer cell lines.
Background: Prominin 2 is a member of the prominin family of pentaspan membrane glycoproteins. The encoded protein localizes to basal epithelial cells and may be involved in the organization of plasma membrane microdomains. Alternative splicing results in multiple transcript variants.Colocalizes with PROM1. Associates with membrane in a cholesterol-dependent manner. Localizes to the apical and basolateral membranes of epithelial cells Present in saliva within small membrane particles (at protein level). Expressed in kidney, prostate, trachea, esophagus, salivary gland, thyroid gland, mammary gland adrenal gland, placenta, stomach, spinal cord and liver. In submucosal tumor, expressed in spindle-shaped or stellate stromal cells. Expressed in prostate cancer cell lines.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
prominin-2 isoform 1
NCBI Official Synonym Full Names
prominin 2
NCBI Official Symbol
Prom2
NCBI Official Synonym Symbols
Prom-rp; AI507243
NCBI Protein Information
prominin-2
UniProt Protein Name
Prominin-2
Protein Family
UniProt Gene Name
Prom2
UniProt Synonym Gene Names
Proml2; PROM-2; mPROML2
UniProt Entry Name
PROM2_MOUSE
Research Articles on PROM2
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Product Notes
The Mouse PROM2 prom2 (Catalog #AAA9717256) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717256 ELISA Kit recognizes Mouse PROM2. It is sometimes possible for the material contained within the vial of "Prominin-2 (PROM2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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