Rabbit anti-Human Programmed cell death protein 4 Polyclonal Antibody | anti-PDCD4 antibody

Rabbit anti-human Programmed cell death protein 4 polyclonal Antibody(PDCD4)

Cat. Num. AAA1493915

• Gene Names: PDCD4; H731
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Antigen Affinity Purified
• Synonyms: Programmed cell death protein 4; Polyclonal Antibody; Rabbit anti-human Programmed cell death protein 4 polyclonal Antibody(PDCD4); Neoplastic transformation inhibitor protein; Nuclear antigen H731-like; Protein 197/15a; PDCD4; H731; anti-PDCD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Sequence Length: 469

• Applicable Applications for anti-PDCD4 antibody: ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Recommended dilution: WB:1:500-5000
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Programmed cell death protein 4 protein (1-260aa)
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-27122 / sc-27123 / sc-292504
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: Programmed cell death protein 4 antibody at 5ug/mlLane 1:Hela whole cell lysateLane 2:MCF-7 whole cell lysateLane 3:jurkat whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 52,51 kDaObserved band size: 52 kDa)

Immunoprecipitation (IP)

(Immunoprecipitating PDCD4 in Hela whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1493915 in Hela whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1493915(4ug)+ Hela whole cell lysate(500ug)Lane 3: Hela whole cell lysate (20ug))

Related Product Information for anti-PDCD4 antibody

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.

NCBI and Uniprot Product Information

• NCBI GI #: 21735596
• NCBI GeneID: 27250
• NCBI Accession #: NP_055271.2
• NCBI GenBank Nucleotide #: NM_014456.4
• UniProt Accession #: Q53EL6; O15501; Q5VZS6; Q6PJI5; Q8TAR5; Q99834; B2RCV4; B5ME91
• Molecular Weight: 50,576 Da
• NCBI Official Full Name: programmed cell death protein 4 isoform 1
• NCBI Official Synonym Full Names: programmed cell death 4 (neoplastic transformation inhibitor)
• NCBI Official Symbol: PDCD4
• NCBI Official Synonym Symbols: H731
• NCBI Protein Information: programmed cell death protein 4
• UniProt Protein Name: Programmed cell death protein 4
• Protein Family: Programmed cell death protein
• UniProt Gene Name: PDCD4
• UniProt Synonym Gene Names: H731
• UniProt Entry Name: PDCD4_HUMAN

NCBI Description

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

Uniprot Description

PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.

Protein type: Tumor suppressor; Apoptosis

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: apoptosis; cell aging; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of JNK activity; negative regulation of transcription, DNA-dependent