Western Blot (WB)
(Western Blot)
anti-Mouse CD16 Polyclonal Antibody | anti-CD16 antibody
Rabbit Anti-CD16
Reactivity
Mouse
Applications
ELISA, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Affinity Purified. Purified by affinity chromatography.
Synonyms
CD16; Polyclonal Antibody; Rabbit Anti-CD16; CD16A; CD16a Antigen; Fc gamma R3; Fc gamma RIII alpha; Fc gamma RIII; Fc gamma RIIIa; FCRIIIA; FCGRIII; FCG3; FCGR3A; FCGR3A Protein; FcR10; FcR-10; FcRIII; FcRIIIa; IGFR3; IgG Fc Receptor III-2; Low Affinity Immunoglobulin gamma Fc Region Receptor III-A; anti-CD16 antibody
Host
Host: Rabbit; Source: Mouse
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Fc Fragment Of IgG Low Affinity IIIa Receptor (FcgR3A)
Purity/Purification
Affinity Purified. Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-CD16 antibody
ELISA (EIA), Immunohistochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 1:100-1:5000 Western Blot: 1:50-1:400 Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (Paraffin): 1:10-1:100 Immunocytochemistry: 1:50-1:500
Included Protein Molecular Weight Marker
168631: Unstained Protein Molecular Weight Marker, 10-70kD Suppled as a liquid in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Ready to use; no need to heat, dilute or add reducing agents before use
Protein Bands
10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD and 70kD. Double Intesity Bands: The 26kD, 18kD and 10kD bands are at double intensity to make location and size approximation of proteins easier.
Immunogen
Recombinant protein corresponding to aa5-240 with two N-terminal Tags, His-tag and GST-tag from mouse CD16, expressed in E. coli
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot)
Western Blot (WB)
(Western Blot)
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Product Notes
The CD16 (Catalog #AAA6120639) is an Antibody produced from Host: Rabbit; Source: Mouse and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-CD16 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD16 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (ICC), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 1:100-1:5000 Western Blot: 1:50-1:400 Immunohistochemistry (frozen): 1:50-1:500 Immunohistochemistry (Paraffin): 1:10-1:100 Immunocytochemistry: 1:50-1:500. Researchers should empirically determine the suitability of the CD16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD16, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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