Western Blot (WB)
Rabbit anti-Human, mouse PRMT5 Polyclonal Antibody | anti-PRMT5 antibody
Anti-PRMT5 antibody
Reactivity
Human, mouse
Applications
Western Blot, Immunocytochemistry
Purity
Immunogen affinity purified
Synonyms
PRMT5; Polyclonal Antibody; Anti-PRMT5 antibody; Polyclonal Anti- PRMT5; anti-PRMT5 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified
Form/Format
Liquid
Applicable Applications for anti-PRMT5 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
WB (1:500); ICC (1:50)
Immunogen
A synthetic peptide (conjugated to KLH) corresponding to the region of PRMT5.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Preparation and Storage
Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles.
Western Blot (WB)
Western Blot (WB)
Testing Data
Testing Data
Related Product Information for anti-PRMT5 antibody
PRMT5 belongs to the methyltransferase enzyme family. It is localized predominantly to the cytoplasm of transfected COS-1 cells and human Chang liver cells.[1]It plays key roles in morphologic control and mating response pathways. Branscombe et al. (2001) showed that human PRMT5 catalyzed the formation of monomethylarginine and symmetric dimethylarginine in a variety of proteins. [2] PRMT5 binding was essential for assembly of a repressive multiprotein complex on the gamma-globin promoter. [3]
References
1. Rho, J., Choi, S., Seong, Y. R., Cho, W.-K., Kim, S. H., Im, D.-S. PRMT5, which forms distinct homo-oligomers, is a member of the protein-arginine methyltransferase family. J. Biol. Chem. 276: 11393-11401, 2001. 2. Branscombe, T. L., Frankel, A., Lee, J.-H., Cook, J. R., Yang, Z., Pestka, S., Clarke, S. PRMT5 (Janus kinase-binding protein 1) catalyzes the formation of symmetric dimethylarginine residues in proteins. J. Biol. Chem. 276: 32971-32976, 2001. 3. Rank, G., Cerruti, L., Simpson, R. J., Moritz, R. L., Jane, S. M., Zhao, Q. Identification of a PRMT5-dependent repressor complex linked to silencing of human fetal globin gene expression. Blood 116: 1585-1592, 2010.
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Product Notes
The PRMT5 (Catalog #AAA355145) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PRMT5 antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRMT5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). WB (1:500); ICC (1:50). Researchers should empirically determine the suitability of the PRMT5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRMT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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