Western Blot (WB)
Rabbit Mono-methyl-Histone H3 / Lys4 Polyclonal Antibody
Anti-Mono-methyl-Histone H3 / Lys4 antibody
Reactivity
Human, mouse, rat
Applications
Western Blot, Immunocytochemistry
Purity
Immunogen affinity purified
Synonyms
Mono-methyl-Histone H3 / Lys4; Polyclonal Antibody; Anti-Mono-methyl-Histone H3 / Lys4 antibody; Polyclonal Anti- Mono-methyl-Histone H3 / Lys4; anti-Mono-methyl-Histone H3 / Lys4 antibody
Host
Rabbit
Reactivity
Human, mouse, rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified
Form/Format
Liquid
Applicable Applications for anti-Mono-methyl-Histone H3 / Lys4 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
WB (1:2000); ICC (1:50)
Immunogen
A synthetic peptide (conjugated to KLH) corresponding to the region of Mono-methyl-Histone H3.
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide
Storage Buffer
In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
Preparation and Storage
Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles
Western Blot (WB)
Western Blot (WB)
Testing Data
Testing Data
Related Product Information for anti-Mono-methyl-Histone H3 / Lys4 antibody
Histones are the basic nuclear proteins responsible for the nucleosome structure within the chromosomal fiber in eukaryotes. Five classes of histone genes have been reported, Histone H3 is one of them.[1] Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. It is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.[2]
References
1. Bhasin M, Reinherz EL, Reche PA (2006). "Recognition and classification of histones using support vector machine". J. Comput. Biol. 13 (1): 102-12. 2. Fischle, W., Tseng, B. S., Dormann, H. L., Ueberheide, B. M., Garcia, B. A., Shabanowitz, J., Hunt, D. F., Funabiki, H., Allis, C. D. Regulation of HP1-chromatin binding by histone H3 methylation and phosphorylation. Nature 438: 1116-1122, 2005
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Product Notes
The Mono-methyl-Histone H3 / Lys4 (Catalog #AAA355139) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mono-methyl-Histone H3 / Lys4 antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's Mono-methyl-Histone H3 / Lys4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). WB (1:2000); ICC (1:50). Researchers should empirically determine the suitability of the Mono-methyl-Histone H3 / Lys4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mono-methyl-Histone H3 / Lys4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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