Rabbit anti-Human Frizzled 5 Polyclonal Antibody | anti-FZD5 antibody

Anti-Frizzled 5 antibody

Cat. Num. AAA355190

• Gene Names: FZD5; HFZ5; C2orf31
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry
• Purity: Immunogen affinity purified
• Synonyms: Frizzled 5; Polyclonal Antibody; Anti-Frizzled 5 antibody; Polyclonal Anti- Frizzled 5; anti-FZD5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified
• Form/Format: Liquid

• Applicable Applications for anti-FZD5 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB (1:500-1000); ICC (1:100-200)
• Immunogen: A synthetic peptide (conjugated with KLH) corresponding to the region of human Frizzled 5.
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide
• Storage Buffer: In TBS (pH7.4), 0.5% BSA, 40% Glycerol and 0.05% Sodium Azide.
• Preparation and Storage: Store at 4 degree C after thawing (1 week). Aliquot and store at -20 degree C for long term (at least one year). Avoid repeated freeze and thaw cycles.

Western Blot (WB)

Related Product Information for anti-FZD5 antibody

Frizzled-5 (FZD5) is a protein that in humans is encoded by the FZD5 gene, this gene encodes a polypeptide of 585 amino acids. [1] It is a member of of the 'frizzled' gene family, which encode 7-transmembrane domain proteins. He et al. (1997) showed that human frizzled 5 is the receptor for the Wnt5A ligand. [2]

References

1. Wang, Y., Macke, J. P., Abella, B. S., Andreasson, K., Worley, P., Gilbert, D. J., Copeland, N. G., Jenkins, N. A., Nathans, J. A large family of putative transmembrane receptors homologous to the product of the Drosophila tissue polarity gene frizzled. J. Biol. Chem. 271: 4468-4476, 1996. 2. He, X., Saint-Jeannet, J.-P., Wang, Y., Nathans, J., Dawid, I., Varmus, H. A member of the frizzled protein family mediating axis induction by Wnt-5A. Science 275: 1652-1654, 1997

NCBI and Uniprot Product Information

• NCBI GI #: 27894385
• NCBI GeneID: 7855
• NCBI Accession #: NP_003459.2
• NCBI GenBank Nucleotide #: NM_003468.3
• UniProt Accession #: Q13467; Q53R22
• Molecular Weight: 64,507 Da
• NCBI Official Full Name: frizzled-5
• NCBI Official Synonym Full Names: frizzled family receptor 5
• NCBI Official Symbol: FZD5
• NCBI Official Synonym Symbols: HFZ5; C2orf31
• NCBI Protein Information: frizzled-5; fz-5; fzE5; Wnt receptor; seven-transmembrane receptor frizzled-5; frizzled 5, seven transmembrane spanning receptor
• UniProt Protein Name: Frizzled-5
• Protein Family: Frizzled
• UniProt Gene Name: FZD5
• UniProt Synonym Gene Names: C2orf31; Fz-5; hFz5
• UniProt Entry Name: FZD5_HUMAN

NCBI Description

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway. Ref.6

Subunit structure: Interacts with GOPC

By similarity.

Subcellular location: Cell membrane; Multi-pass membrane protein

By similarity. Golgi apparatus membrane; Multi-pass membrane protein

By similarity. Note: Localized at the plasma membrane and also found at the Golgi

By similarity. Ref.6

Domain: The PDZ-binding motif mediates interaction with GOPC

By similarity.Lys-Thr-X-X-X-Trp motif interacts with the PDZ doman of Dvl (Disheveled) family members and is involved in the activation of the Wnt/beta-catenin signaling pathway

By similarity.The FZ domain is involved in binding with Wnt ligands

By similarity.

Post-translational modification: Ubiquitinated by RNF43 and ZNRF3, leading to its degradation by the proteasome. Ref.7 Ref.8

Sequence similarities: Belongs to the G-protein coupled receptor Fz/Smo family.Contains 1 FZ (frizzled) domain.