Rabbit anti-Mouse Prkcz Polyclonal Antibody | anti-Prkcz antibody

Prkcz, NT (Prkcz, Pkcz, Protein kinase C zeta type, nPKC-zeta) (PE)

Cat. Num. AAA6337390

• Gene Names: Prkcz; Pkcz; C80388; R74924; zetaPKC; AI098070; aPKCzeta; nPKC-zeta
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Prkcz; Polyclonal Antibody; NT (Prkcz; Pkcz; Protein kinase C zeta type; nPKC-zeta) (PE); nPKC-zeta; anti-Prkcz antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Prkcz antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Prkcz antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of Mouse Prkcz.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Prkcz Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Prkcz antibody detected the Prkcz protein (arrow).)

Related Product Information for anti-Prkcz antibody

This is a calcium-independent, phospholipid-dependent, serine-and threonine-specific enzyme. Phosphorylates KCNAB2. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.

Product Categories/Family for anti-Prkcz antibody

Antibodies; Protein Kinases

References

Lee, S.J., et al. Cell Metab. 12(1):65-77(2010). Gao, N., et al. Genes Dev. 24(12):1295-1305(2010). Yates, L.L., et al. Hum. Mol. Genet. 19(11):2251-2267(2010). Kim, S., et al. Neuron 66(1):69-84(2010). de Thonel, A., et al. Mol. Biol. Cell 21(8):1423-1434(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 84872200
• NCBI GeneID: 18762
• NCBI Accession #: NP_032886.2
• NCBI GenBank Nucleotide #: NM_008860.3
• UniProt Accession #: Q02956; A2AD76
• Molecular Weight: 67,682 Da
• NCBI Official Full Name: protein kinase C zeta type isoform a
• NCBI Official Synonym Full Names: protein kinase C, zeta
• NCBI Official Symbol: Prkcz
• NCBI Official Synonym Symbols: Pkcz; C80388; R74924; zetaPKC; AI098070; aPKCzeta; nPKC-zeta
• NCBI Protein Information: protein kinase C zeta type; atypical protein kinase C; protein kinase C zeta subspecies
• UniProt Protein Name: Protein kinase C zeta type
• Protein Family: Protein kinase
• UniProt Gene Name: Prkcz
• UniProt Synonym Gene Names: Pkcz
• UniProt Entry Name: KPCZ_MOUSE

Uniprot Description

PKCZ: an AGC kinase of the PKC family. An atypical PKC: its activity is not regulated by Ca2+, PS, DAG or phorbol esters. Constitutively active.

Protein type: Protein kinase, AGC; Kinase, protein; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); AGC group; PKC family; Iota subfamily

Cellular Component: tight junction; nuclear matrix; protein complex; intracellular membrane-bound organelle; apical cortex; leading edge; nuclear envelope; intercellular junction; cell cortex; cytosol; lipid raft; axon hillock; filamentous actin; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; plasma membrane; microtubule organizing center; cell junction; nucleus; endosome

Molecular Function: protein domain specific binding; protein kinase C activity; metal ion binding; nucleotide binding; protein kinase binding; protein kinase activity; transferase activity; protein serine/threonine kinase activity; protein binding; phospholipase binding; potassium channel regulator activity; transferase activity, transferring phosphorus-containing groups; kinase activity; ATP binding

Biological Process: protein heterooligomerization; membrane hyperpolarization; negative regulation of insulin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; positive regulation of synaptic transmission; activation of NF-kappaB transcription factor; positive regulation of interleukin-4 production; positive regulation of glucose import; positive regulation of T-helper 2 cell differentiation; cell surface receptor linked signal transduction; negative regulation of hydrolase activity; positive regulation of cell proliferation; vesicle transport along microtubule; inflammatory response; positive regulation of cell-matrix adhesion; negative regulation of peptidyl-tyrosine phosphorylation; phospholipase D activation; cell migration; activation of protein kinase B; microtubule cytoskeleton organization and biogenesis; positive regulation of protein transport; membrane depolarization; peptidyl-serine phosphorylation; long-term memory; cellular response to insulin stimulus; establishment of cell polarity; actin cytoskeleton reorganization; insulin receptor signaling pathway; positive regulation of insulin receptor signaling pathway; negative regulation of protein complex assembly; phosphorylation; negative regulation of apoptosis