Mouse PKCz ELISA Kit | PKCz elisa kit

Mouse PKCz (Protein Kinase C Zeta) ELISA Kit

Cat. Num. AAA2503937

• Gene Names: Prkcz; Pkcz; C80388; R74924; zetaPKC; AI098070; aPKCzeta; nPKC-zeta
• Reactivity: Mouse
• Synonyms: PKCz; Mouse PKCz (Protein Kinase C Zeta) ELISA Kit; PKCz elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes natural and recombinant Mouse PKCz. No significant cross-reactivity or interference between Mouse PKCz and analogues was observed.
• Sequence Length: 409

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PKCz elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse PKCz concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PKCz. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PKCz and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PKCz, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PKCz. You can calculate the concentration of PKCz in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 84872205
• NCBI GeneID: 18762
• NCBI Accession #: NP_001034168.1
• NCBI GenBank Nucleotide #: NM_001039079.2
• UniProt Accession #: Q02956; A2AD76
• Molecular Weight: 67,682 Da
• NCBI Official Full Name: protein kinase C zeta type isoform b
• NCBI Official Synonym Full Names: protein kinase C, zeta
• NCBI Official Symbol: Prkcz
• NCBI Official Synonym Symbols: Pkcz; C80388; R74924; zetaPKC; AI098070; aPKCzeta; nPKC-zeta
• NCBI Protein Information: protein kinase C zeta type; atypical protein kinase C; protein kinase C zeta subspecies
• UniProt Protein Name: Protein kinase C zeta type
• UniProt Gene Name: Prkcz
• UniProt Synonym Gene Names: Pkcz
• UniProt Entry Name: KPCZ_MOUSE

Uniprot Description

PKCZ: an AGC kinase of the PKC family. An atypical PKC: its activity is not regulated by Ca2+, PS, DAG or phorbol esters. Constitutively active.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.13; Protein kinase, AGC; AGC group; PKC family; Iota subfamily

Cellular Component: tight junction; nuclear matrix; protein complex; apical cortex; intracellular membrane-bound organelle; leading edge; nuclear envelope; cell cortex; intercellular junction; cytosol; lipid raft; axon hillock; filamentous actin; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; plasma membrane; microtubule organizing center; cell junction; nucleus; endosome

Molecular Function: transferase activity; protein domain specific binding; protein serine/threonine kinase activity; protein binding; potassium channel regulator activity; protein kinase C activity; phospholipase binding; metal ion binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: protein heterooligomerization; membrane hyperpolarization; negative regulation of insulin receptor signaling pathway; signal transduction; protein amino acid phosphorylation; positive regulation of synaptic transmission; activation of NF-kappaB transcription factor; positive regulation of interleukin-4 production; positive regulation of glucose import; cell surface receptor linked signal transduction; positive regulation of T-helper 2 cell differentiation; negative regulation of hydrolase activity; positive regulation of cell proliferation; vesicle transport along microtubule; inflammatory response; positive regulation of cell-matrix adhesion; phospholipase D activation; negative regulation of peptidyl-tyrosine phosphorylation; cell migration; activation of protein kinase B; microtubule cytoskeleton organization and biogenesis; positive regulation of protein transport; membrane depolarization; peptidyl-serine phosphorylation; cellular response to insulin stimulus; long-term memory; establishment of cell polarity; actin cytoskeleton reorganization; insulin receptor signaling pathway; positive regulation of insulin receptor signaling pathway; phosphorylation; negative regulation of protein complex assembly; negative regulation of apoptosis