Rabbit anti-Mouse Prkce Polyclonal Antibody | anti-Prkce antibody

Prkce, NT (Prkce, Pkce, Pkcea, Protein kinase C epsilon type, nPKC-epsilon) (AP)

Cat. Num. AAA6337336

• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Prkce; Polyclonal Antibody; NT (Prkce; Pkce; Pkcea; Protein kinase C epsilon type; nPKC-epsilon) (AP); nPKC-epsilon; anti-Prkce antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-Prkce antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Prkce antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-150 amino acids from the N-terminal region of Mouse Prkce.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Prkce Antibody (N-term) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the Prkce antibody detected the Prkce protein (arrow).)

Related Product Information for anti-Prkce antibody

This is calcium-independent, phospholipid-dependent, serine-and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Product Categories/Family for anti-Prkce antibody

Antibodies; Protein Kinases

References

Chou, W.H., et al. J. Neurosci. 30(42):13955-13965(2010). Roh, D.H., et al. Neurosci. Lett. 477(2):95-99(2010). Batarseh, A., et al. Biochemistry 49(23):4766-4778(2010). Fenton, R.A., et al. Am. J. Physiol. Heart Circ. Physiol. 298 (6), H1671-H1678 (2010): Gan, L., et al. Mol. Immunol. 47(6):1278-1282(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 6755084
• NCBI GeneID: 18754
• NCBI Accession #: NP_035234.1
• NCBI GenBank Nucleotide #: NM_011104.3
• UniProt Accession #: P16054
• Molecular Weight: 83,561 Da
• NCBI Official Full Name: protein kinase C epsilon type
• UniProt Protein Name: Protein kinase C epsilon type
• Protein Family: Protein kinase
• UniProt Gene Name: Prkce
• UniProt Synonym Gene Names: Pkce; Pkcea
• UniProt Entry Name: KPCE_MOUSE

Uniprot Description

PKCE: an AGC kinase of the PKC family. Calcium-independent, phospholipid-dependent. Activated by inflammatory mediators and involved in nociceptive functions. PKC-epsilon null mice display decreased hypoxic pulmonary vasoconstriction.

Protein type: EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; AGC group; PKC family; Eta subfamily

Cellular Component: cytoplasm; cytoskeleton; cytosol; endoplasmic reticulum; Golgi apparatus; Golgi membrane; membrane; mitochondrion; nucleus; perinuclear region of cytoplasm; plasma membrane

Molecular Function: actin monomer binding; ATP binding; calcium-independent protein kinase C activity; enzyme activator activity; enzyme binding; ethanol binding; kinase activity; metal ion binding; nucleotide binding; protein binding; protein kinase activity; protein kinase binding; protein kinase C activity; protein serine/threonine kinase activity; receptor activator activity; receptor binding; SH3 domain binding; transferase activity

Biological Process: cell adhesion; cell cycle; cell division; chemosensory behavior; immune system process; lipopolysaccharide-mediated signaling pathway; macrophage activation during immune response; negative regulation of apoptosis; negative regulation of protein ubiquitination; negative regulation of release of sequestered calcium ion into cytosol; peptidyl-serine phosphorylation; phosphorylation; positive regulation of actin filament polymerization; positive regulation of cytokinesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of insulin secretion; positive regulation of lipid catabolic process; positive regulation of MAPKKK cascade; positive regulation of synaptic transmission, GABAergic; protein amino acid phosphorylation; reduction of mitochondrial calcium ion concentration; regulation of lipid metabolic process; regulation of peptidyl-tyrosine phosphorylation; regulation of release of sequestered calcium ion into cytosol; release of sequestered calcium ion into cytosol; response to morphine