Principle of the Assay: MDA in the catabolite of lipid peroxide can react with thiobarbituric acid (TBA) and produce red compound, which has a maximum absorption peak at 532 nm.
Background/Introduction: The body produce oxygen free radicals through the enzyme system and nonenzyme system, which can attack unsaturated fatty acid on biofilm and lead to lipid peroxidation and form lipid peroxide, such as aldehyde group (MDA), keto-, hydroxyl, carbonyl, etc. Oxygen free radicals cause cell damage not only by peroxidation of polyunsaturated fatty acids in biofilm, but also by decomposition products of lipid hydroperoxide. Detection of the MDA content can reflect the level of lipid peroxidation in cells and reflect level of cellular damage indirectly.
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Product Notes
The MDA (Catalog #AAA2567678) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Malondialdehyde (MDA), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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