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Flow Cytometry (FC/FACS) (Flow Cytometric analysis of Hela cells using NEU2 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Mouse NEU2 Monoclonal Antibody | anti-NEU2 antibody

NEU2 antibody

Gene Names
NEU2; SIAL2
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
NEU2 antibody was purified by affinity chromatography.
Synonyms
NEU2; Monoclonal Antibody; NEU2 antibody; Monoclonal NEU2; Anti-NEU2; Sialidase 2; Cytosolic sialidase; SIAL2; N-acetyl-alpha-neuraminidase 2; Neuraminidase 2; NEU 2; NEU-2; anti-NEU2 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
4F4
Purity/Purification
NEU2 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
380
Applicable Applications for anti-NEU2 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
WB: 1:2000
Biological Significance
NEU2 belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosoliclocalization was demonstrated by cell fractionation experiments.
Cross-Reactivity
Human
Immunogen
NEU2 antibody was raised in mouse using a full length recombinant protein of human NEU2 (NP_005374) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Hela cells using NEU2 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS) (Flow Cytometric analysis of Hela cells using NEU2 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NEU2 protein (Right) or empty vector (Left) detected with NEU2 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant NEU2 protein (Right) or empty vector (Left) detected with NEU2 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant NEU2 protein using NEU2 antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant NEU2 protein using NEU2 antibody)
Related Product Information for anti-NEU2 antibody
Mouse monoclonal NEU2 antibody
Product Categories/Family for anti-NEU2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,254 Da
NCBI Official Full Name
sialidase-2
NCBI Official Synonym Full Names
sialidase 2 (cytosolic sialidase)
NCBI Official Symbol
NEU2
NCBI Official Synonym Symbols
SIAL2
NCBI Protein Information
sialidase-2
UniProt Protein Name
Sialidase-2
Protein Family
UniProt Gene Name
NEU2
UniProt Entry Name
NEUR2_HUMAN

NCBI Description

This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq, Jul 2008]

Uniprot Description

NEU2: Hydrolyzes sialylated compounds. Belongs to the glycosyl hydrolase 33 family.

Protein type: Glycan Metabolism - other glycan degradation; EC 3.2.1.18; Hydrolase; Lipid Metabolism - sphingolipid

Chromosomal Location of Human Ortholog: 2q37

Cellular Component: cytoplasm; cytosol

Biological Process: oligosaccharide catabolic process; cellular protein metabolic process; sphingolipid metabolic process; dolichol-linked oligosaccharide biosynthetic process; ganglioside catabolic process; protein amino acid N-linked glycosylation via asparagine; glycosphingolipid metabolic process; post-translational protein modification

Research Articles on NEU2

Similar Products

Product Notes

The NEU2 neu2 (Catalog #AAA833871) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NEU2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC: 1:100 IF: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the NEU2 neu2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEU2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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