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Western Blot (WB) (Western blot analysis of anti-PRKAR2B Pab in mouse brain tissue lysate. PRKAR2B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Rabbit anti-Human, Mouse PRKAR2B Polyclonal Antibody | anti-PRKAR2B antibody

PRKAR2B, NT (cAMP-dependent Protein Kinase Type II-beta Regulatory Subunit) (AP)

Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
PRKAR2B; Polyclonal Antibody; NT (cAMP-dependent Protein Kinase Type II-beta Regulatory Subunit) (AP); anti-PRKAR2B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human PRKAR2B. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-PRKAR2B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human PRKAR2B (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PRKAR2B Pab in mouse brain tissue lysate. PRKAR2B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB) (Western blot analysis of anti-PRKAR2B Pab in mouse brain tissue lysate. PRKAR2B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Western Blot (WB)

(Western blot analysis of PRKAR2B (arrow) using rabbit polyclonal PRKAR2B Antibody (G46). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRKAR2B gene.)

Western Blot (WB) (Western blot analysis of PRKAR2B (arrow) using rabbit polyclonal PRKAR2B Antibody (G46). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRKAR2B gene.)

Western Blot (WB)

(PRKAR2B Antibody (G46) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the PRKAR2B antibody detected the PRKAR2B protein (arrow).)

Western Blot (WB) (PRKAR2B Antibody (G46) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the PRKAR2B antibody detected the PRKAR2B protein (arrow).)
Product Categories/Family for anti-PRKAR2B antibody
References
1. Levy, F.O., et al., Mol. Endocrinol. 2(12):1364-1373 (1988).

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Product Notes

The PRKAR2B (Catalog #AAA6495081) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRKAR2B, NT (cAMP-dependent Protein Kinase Type II-beta Regulatory Subunit) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRKAR2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-1:500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PRKAR2B for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRKAR2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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