Rabbit anti-Human PRKAR2B Polyclonal Antibody | anti-PRKAR2B antibody

PRKAR2B, ID (PRKAR2B, cAMP-dependent protein kinase type II-beta regulatory subunit) (APC)

Cat. Num. AAA6337293

• Gene Names: PRKAR2B; PRKAR2; RII-BETA
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PRKAR2B; Polyclonal Antibody; ID (PRKAR2B; cAMP-dependent protein kinase type II-beta regulatory subunit) (APC); cAMP-dependent protein kinase type II-beta regulatory subunit; anti-PRKAR2B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PRKAR2B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PRKAR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-147 amino acids from the Central region of human PRKAR2B.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of PRKAR2B (arrow) using rabbit polyclonal PRKAR2B Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRKAR2B gene.)

Related Product Information for anti-PRKAR2B antibody

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol. [provided by RefSeq].

Product Categories/Family for anti-PRKAR2B antibody

Antibodies; Protein Kinases

References

Liu, Y.J., et al. Obesity (Silver Spring) (2010). In press: Adkins, D.E., et al. Mol. Psychiatry (2010). In press: Islam, A., et al. J. Biol. Chem. 283(37):25364-25371(2008). Ji, Z., et al. J. Biol. Chem. 283(32):21920-21925(2008). Vincent-Dejean, C., et al. Eur. J. Endocrinol. 158(6):829-839(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 47132585
• NCBI GeneID: 5577
• NCBI Accession #: NP_002727.2
• NCBI GenBank Nucleotide #: NM_002736.2
• UniProt Accession #: P31323; A4D0R9
• Molecular Weight: 46,302 Da
• NCBI Official Full Name: cAMP-dependent protein kinase type II-beta regulatory subunit
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, regulatory, type II, beta
• NCBI Official Symbol: PRKAR2B
• NCBI Official Synonym Symbols: PRKAR2; RII-BETA
• NCBI Protein Information: cAMP-dependent protein kinase type II-beta regulatory subunit; H_RG363E19.2; WUGSC:H_RG363E19.2; cAMP-dependent protein kinase type II-beta regulatory chain
• UniProt Protein Name: cAMP-dependent protein kinase type II-beta regulatory subunit
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKAR2B
• UniProt Entry Name: KAP3_HUMAN

NCBI Description

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activated T cells. Knockout studies in mice suggest that this subunit may play an important role in regulating energy balance and adiposity. The studies also suggest that this subunit may mediate the gene induction and cataleptic behavior induced by haloperidol. [provided by RefSeq, Jul 2008]

Uniprot Description

PKAR2B: a regulatory subunit of cAMP-regulated protein kinase. The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Protein type: Mitochondrial; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: centrosome; cytoplasm; dendritic spine; plasma membrane; dendritic shaft; cytosol; cAMP-dependent protein kinase complex

Molecular Function: ubiquitin protein ligase binding; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; organelle organization and biogenesis; activation of protein kinase A; learning; fatty acid metabolic process; signal transduction; phospholipase C activation; energy reserve metabolic process; innate immune response; renal water homeostasis; mitotic cell cycle; blood coagulation; G2/M transition of mitotic cell cycle; transmembrane transport; regulation of insulin secretion