Rabbit anti-Human, Mouse PRKAR1B Polyclonal Antibody | anti-PRKAR1B antibody

PRKAR1B, NT (cAMP-dependent Protein Kinase Type I-beta Regulatory Subunit)

Cat. Num. AAA630376

• Gene Names: PRKAR1B; PRKAR1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PRKAR1B; Polyclonal Antibody; NT (cAMP-dependent Protein Kinase Type I-beta Regulatory Subunit); Anti -PRKAR1B; anti-PRKAR1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRKAR1B. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-PRKAR1B antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunohistochemistry.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500; Immunohistochemistry: 1:50-1:100
• Immunogen: Synthetic peptide selected from the N-terminal region of human PRKAR1B (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PRKAR1B Pab in mouse liver tissue lysate. PRKAR1B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-PRKAR1B antibody

The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Ca, Cb and Cgamma, that each represent specific gene products. Ca and Cb are closely related (93% amino acid sequence similari- ty), whereas Cgamma displays 83% and 79% similarity to Ca and Cb, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcrip- tion factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.

Product Categories/Family for anti-PRKAR1B antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 62020783
• NCBI GeneID: 5575
• UniProt Accession #: P31321; Q8N422; Q6NS41
• Molecular Weight: 43,073 Da
• NCBI Official Full Name: PRKAR1B protein
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, regulatory, type I, beta
• NCBI Official Symbol: PRKAR1B
• NCBI Official Synonym Symbols: PRKAR1
• NCBI Protein Information: cAMP-dependent protein kinase type I-beta regulatory subunit; OTTHUMP00000199947; OTTHUMP00000199948; OTTHUMP00000199949; OTTHUMP00000199952
• UniProt Protein Name: cAMP-dependent protein kinase type I-beta regulatory subunit
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKAR1B
• UniProt Entry Name: KAP1_HUMAN

NCBI Description

Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.[supplied by OMIM]

Uniprot Description

PKAR1B: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase. Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Belongs to the cAMP-dependent kinase regulatory chain family.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: plasma membrane; cytosol; cAMP-dependent protein kinase complex

Molecular Function: cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; signal transduction; protein amino acid phosphorylation; learning and/or memory; phospholipase C activation; energy reserve metabolic process; renal water homeostasis; innate immune response; blood coagulation; transmembrane transport; regulation of insulin secretion