Rabbit anti-Human, Mouse PRKAR1B Polyclonal Antibody | anti-PRKAR1B antibody

PRKAR1B, NT (cAMP-dependent Protein Kinase Type I-beta Regulatory Subunit) (FITC)

Cat. Num. AAA6495073

• Gene Names: PRKAR1B; PRKAR1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: PRKAR1B; Polyclonal Antibody; NT (cAMP-dependent Protein Kinase Type I-beta Regulatory Subunit) (FITC); anti-PRKAR1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PRKAR1B. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PRKAR1B antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human PRKAR1B (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-PRKAR1B Pab in mouse liver tissue lysate. PRKAR1B (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-PRKAR1B antibody

Antibodies; Protein Kinases

References

1. Solberg, R., et al., Exp. Cell Res. 214(2):595-605 (1994). 2. Solberg, R., et al., Biochem. Biophys. Res. Commun. 176(1):166-172 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 38257139
• NCBI GeneID: 5575
• NCBI Accession #: NP_002726
• NCBI GenBank Nucleotide #: NM_002735.2
• UniProt Accession #: P31321; Q8N422
• Molecular Weight: 43,073 Da
• NCBI Official Full Name: cAMP-dependent protein kinase type I-beta regulatory subunit
• NCBI Official Synonym Full Names: protein kinase, cAMP-dependent, regulatory, type I, beta
• NCBI Official Symbol: PRKAR1B
• NCBI Official Synonym Symbols: PRKAR1
• NCBI Protein Information: cAMP-dependent protein kinase type I-beta regulatory subunit
• UniProt Protein Name: cAMP-dependent protein kinase type I-beta regulatory subunit
• Protein Family: cAMP-dependent protein kinase
• UniProt Gene Name: PRKAR1B
• UniProt Entry Name: KAP1_HUMAN

NCBI Description

Cyclic AMP-dependent protein kinase A (PKA) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription. The PKA holoenzyme is composed of 2 regulatory and 2 catalytic subunits and dissociates from the regulatory subunits upon binding of cAMP.[supplied by OMIM, Jun 2009]

Uniprot Description

PKAR1B: Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase. Four types of regulatory chains are found: I- alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. Belongs to the cAMP-dependent kinase regulatory chain family.

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: plasma membrane; cytosol; cAMP-dependent protein kinase complex

Molecular Function: cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; cAMP binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; signal transduction; protein amino acid phosphorylation; learning and/or memory; phospholipase C activation; energy reserve metabolic process; innate immune response; renal water homeostasis; blood coagulation; regulation of insulin secretion; transmembrane transport