Rabbit Precerebellin Polyclonal Antibody | anti-CBLN1 antibody

Precerebellin Antibody

Cat. Num. AAA150458

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Precerebellin Antibody is affinity chromatography purified via peptide column.
• Synonyms: Precerebellin; Polyclonal Antibody; Precerebellin Antibody; Cerebellin-1; CER; cerebellin 1 precursor; anti-CBLN1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Precerebellin Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 193

• Applicable Applications for anti-CBLN1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Precerebellin antibody can be used for the detection of precerebellin by Western blot at 2 - 4 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Precerebellin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy- terminus human precerebellin.
• Buffer: Precerebellin Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Precerebellin antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of precerebellin in 293 cell lysate with precerebellin antibody at (A) 2 and (B) 4 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of precerebellin in 3T3 cells with precerebellin antibody at 10 μg/mL.)

Related Product Information for anti-CBLN1 antibody

Precerebellin Antibody: Precerebellin is the precursor of the brain-specific hexadecapeptide cerebellin, a protein with substantial similarity to the globular region of the B chain of complement component C1q. Cerebellin exerts neuromodulatory functions by directly stimulating norepinephrine release via the adenylate cyclase/pka- dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release. The active form of precerebellin is highly enriched in postsynaptic structures of cerebellar Purkinje cells in cartwheel neurons of the dorsal cochlear nucleus. Because precerebellin belongs to the C1q/tumor necrosis factor superfamily of secreted proteins and has similarity to adiponectin and CTRP3, it has been suggested that precerebellin posseses functions other than those already stated.

NCBI and Uniprot Product Information

• NCBI GI #: 116114
• NCBI GeneID: 869
• UniProt Accession #: P23435; P02682; Q52M09; B2RAN9
• Molecular Weight: 21,097 Da
• NCBI Official Full Name: Cerebellin-1
• NCBI Official Synonym Full Names: cerebellin 1 precursor
• NCBI Official Symbol: CBLN1
• NCBI Protein Information: cerebellin-1; precerebellin
• UniProt Protein Name: Cerebellin-1
• Protein Family: Cerebellin
• UniProt Gene Name: CBLN1
• UniProt Synonym Gene Names: CER
• UniProt Entry Name: CBLN1_HUMAN

NCBI Description

This gene encodes a cerebellum-specific precursor protein, precerebellin, with similarity to the globular (non-collagen-like) domain of complement component C1qB. Precerebellin is processed to give rise to several derivatives, including the hexadecapeptide, cerebellin, which is highly enriched in postsynaptic structures of Purkinje cells. Cerebellin has also been found in human and rat adrenals, where it has been shown to enhance the secretory activity of this gland. [provided by RefSeq, Aug 2008]

Uniprot Description

CBLN1: Cerebellin-1 is required for synapse integrity and synaptic plasticity. Required for the ER export and secretion of CBLN3.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: postsynaptic membrane; extracellular region; cell junction

Molecular Function: protein homodimerization activity

Biological Process: heterophilic cell adhesion; synaptic transmission; nervous system development; positive regulation of synaptogenesis; protein secretion; cerebellar granule cell differentiation