Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PPP5C Antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Rabbit PPP5C Polyclonal Antibody | anti-PPP5C antibody
PPP5C Polyclonal Antibody
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PPP5C Antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PPP5C Antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]
Uniprot Description
PPP5C: a Ser/Thr phosphatase. Plays a role in the survival of cells in a low oxygen stress by suppressing a hypoxia-induced ASK-1/MKK4/JNK signaling cascade that promotes apoptosis. Has been reported to interact with DNA-PK, dephosphorylating it at two functional sites. May play a role in the regulation of RNA biogenesis and/or mitosis.
Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 19q13.32
Cellular Component: cytosol; intracellular membrane-bound organelle; nucleoplasm; nucleus; protein complex
Molecular Function: ADP binding; ATP binding; Hsp90 protein binding; identical protein binding; phosphoprotein phosphatase activity; protein binding; protein serine/threonine phosphatase activity; signal transducer activity
Biological Process: MAPKKK cascade; mitotic cell cycle; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein amino acid dephosphorylation; transcription, DNA-dependent
Research Articles on PPP5C
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Product Notes
The PPP5C ppp5c (Catalog #AAA9131701) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP5C Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PPP5C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the PPP5C ppp5c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPP5C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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