Mouse CASP7 / Caspase 7 Monoclonal Antibody | anti-CASP7 antibody

Mouse Monoclonal [clone 7-1-11] (IgG) to Human CASP7 / Caspase 7

Cat. Num. AAA246290

• Gene Names: CASP7; MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
• Reactivity: Dog, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein G Purified
• Synonyms: CASP7 / Caspase 7; Monoclonal Antibody; Mouse Monoclonal [clone 7-1-11] (IgG) to Human CASP7 / Caspase 7; Anti-CASP7 / Caspase 7 Antibody (clone 7-1-11) IHC-plus; CASP7; Apoptotic protease Mch-3; Caspase 7; Caspase-7; ICE-LAP3; LICE2; MCH3; ICE-like apoptotic protease 3; CASP-7; CMH-1; Human CASP7; anti-CASP7 antibody

• Host: Mouse
• Reactivity: Dog, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 7-1-11
• Specificity: Detects an ~37 kD protein, corresponding to the apparent molecular mass of the caspase-7 on SDS-PAGE immunoblots, in samples from human, mouse, rat, monkey, dog, sheep, rabbit, pig and hamster origins. This antibody recognizes the unprocessed pro-caspase-7 (p35), caspase-7 lacking the short amino- terminal pro domain (32 kD) and the fully processed caspase-7 (p19) in apoptotic cell extracts.
• Purity/Purification: Protein G Purified
• Form/Format: PBS (10 mM sodium phosphate, pH 7.2, 50 mM sodium chloride, 0.1 mM PMSF). Sourced in Ascites.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 303

• Applicable Applications for anti-CASP7 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Recombinant human caspase-7 full length protein.
• Immunogen Type: Recombinant protein
• Immunogen: CASP7 / Caspase 7 antibody was raised against recombinant human caspase-7 full length protein.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.

Immunohistochemistry (IHC)

(Anti-Caspase 7 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-Caspase 7 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-Caspase 7 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-CASP7 antibody

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens.

Product Categories/Family for anti-CASP7 antibody

Family: Protease; Subfamily: Cysteine C14

NCBI and Uniprot Product Information

• NCBI GI #: 4502581
• NCBI GeneID: 840
• NCBI Accession #: NP_001218.1
• NCBI GenBank Nucleotide #: NM_001227.4
• UniProt Accession #: P55210; Q13364; Q53YD5; Q5SVL0; Q5SVL3; Q96BA0; B4DQU7; B5BU45; D3DRB8
• Molecular Weight: 31,614 Da
• NCBI Official Full Name: caspase-7 isoform alpha
• NCBI Official Synonym Full Names: caspase 7, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP7
• NCBI Official Synonym Symbols: MCH3; CMH-1; LICE2; CASP-7; ICE-LAP3
• NCBI Protein Information: caspase-7; apoptotic protease MCH-3; ICE-like apoptotic protease 3; caspase 7, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-7
• Protein Family: Caspase
• UniProt Gene Name: CASP7
• UniProt Synonym Gene Names: MCH3; CASP-7; ICE-LAP3
• UniProt Entry Name: CASP7_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Uniprot Description

CASP7: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly- 217' bond. Overexpression promotes programmed cell death. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 11 kDa (p11) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. No expression in the brain. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Protease; Apoptosis; EC 3.4.22.60; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: apoptosis; caspase activation via cytochrome c; proteolysis; cell structure disassembly during apoptosis