Rabbit PPP1R1B Polyclonal Antibody | anti-PPP1R1B antibody

Anti-PPP1R1B Antibody, Rabbit Polyclonal

Cat. Num. AAA8108641

• Reactivity: Human, Cynomolgus
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: PPP1R1B; Polyclonal Antibody; Anti-PPP1R1B Antibody; Rabbit Polyclonal; PPP1R1B Antibody; Rabbit PAb; Antigen Affinity Purified; protein phosphatase 1; regulatory (inhibitor) subunit 1B; Anti-DARPP-32 Antibody; Anti-DARPP32 Antibody; anti-PPP1R1B antibody

• Host: Rabbit
• Reactivity: Human, Cynomolgus
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PPP1R1B
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PPP1R1B antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:100-1:400; ICC/IF: 1:100-1:500
• Immunogen: E Coli-derived Human PPP1R1B fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human PPP1R1B fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining PPP1R1B in human colon with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining PPP1R1B in cynomolgus cerebellum with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of PPP1R1B in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human PPP1R1B polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Cytoplasm.)

Related Product Information for anti-PPP1R1B antibody

Dopamine and cAMP-regulated phosphoprotein Mr 32, 000 (DARPP-32), also known as phosphoprotein phosphatase-1 regulatory subunit 1B (PPP1R1B), was initially discovered as a substrate of dopamine-activated protein kinase A (PKA) in the neostriatum in the brain. DARPP-32 plays a central role in the biology of dopamine-receptive neurons; its coding gene (PPP1R1B) has been linked to psychological and psychopathological traits. The PPP1R1B gene is located on chromosome 17q12 (39, 626, 208-39, 636, 626[GRCh38/hg38]), which codes for multiple transcripts and two experimentally-documented proteins Darpp-32 and t-Darpp. The combined evidence from reduced DARPP-32 expression in the dorsolateral prefrontal cortex (DLPFC) in schizophrenic patients and from abnormalities in mice with a genetic deletion of DARPP-32 or with point mutations in phosphorylation sites of DARPP-32 suggested that it would be worthwhile to investigate the association between DARPP-32 and schizophrenia.

Product Categories/Family for anti-PPP1R1B antibody

Phosphatases and Regulators

References

Huang Y, et al. (2013) Association between ppp1r1b polymorphisms and defense mechanisms in healthy chinese-han subjects. J Mol Neurosci 49 (3): 618-624.Hu JX, et al. (2007) An association study between ppp1r1b gene and schizophrenia in the chinese population. Prog Neuropsychopharmacol Biol Psychiatry 31 (6): 1303-1306.Belkhiri A, et al. (2016) Darpp-32: From neurotransmission to cancer. Oncotarget 7 (14): 17631-17640.Avanes A, et al. (2019) Darpp-32 and t-darpp protein products of ppp1r1b: Old dogs with new tricks. Biochem Pharmacol 160 71-79.