anti-Human HAUSP/USP7 Monoclonal Antibody | anti-HAUSP/USP7 antibody

Anti-HAUSP/USP7 Mouse mAb

Cat. Num. AAA475117

• Gene Names: USP7; TEF1; HAUSP
• Reactivity: Human
• Applications: Western Blot
• Synonyms: HAUSP/USP7; Monoclonal Antibody; Anti-HAUSP/USP7 Mouse mAb; Deubiquitinating enzyme 7; HAUSP; herpes virus associated; Herpes virus associated ubiquitin specific protease; Herpesvirus-associated ubiquitin-specific protease; TEF 1; tef-1; TEF1; Ubiquitin carboxyl terminal hydrolase 7; Ubiquitin carboxyl-terminal hydrolase 7; Ubiquitin specific peptidase 7 (herpes virus associated); Ubiquitin specific peptidase 7; Ubiquitin specific peptidase 7 herpes virus associated; Ubiquitin specific processing protease 7; Ubiquitin specific protease 7 (herpes virus associated); Ubiquitin Specific Protease 7; Ubiquitin specific protease 7 herpes virus associated; Ubiquitin thioesterase 7; ubiquitin thiolesterase 7; Ubiquitin-specific-processing protease 7; UBP 7; UBP-7; UBP7; UBP7_HUMAN; USP 7; usp-7; USP7; VMW110-ASSOCIATED PROTEIN; 135-KD; anti-HAUSP/USP7 antibody

• Host: Mouse; Source: Human
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3E4-D10-E8
• Form/Format: Ascites
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1044

• Applicable Applications for anti-HAUSP/USP7 antibody: Western Blot (WB)
• Application Notes: WB: 1:200
• Immunogen: Purified recombinant human HAUSP / USP7 protein fragments expressed in E Coli.
• Sensitivity: This antibody detects endogenous levels of HAUSP / USP7 and does not cross-react with related proteins.
• Sequence Similarities: Belongs to the peptidase C19 family.Contains 1 MATH domain.
• Subunit Structure: Monomer.Homodimer.Part of a complex with DAXX, MDM2, RASSF1 and USP7.Part of a complex with DAXX, MDM2 and USP7.Interacts with MDM2; the interaction is independent of p53/TP53.Interacts with DAXX; the interaction is direct and independent of MDM2 and p53/TP53.Interacts with FOXO4; the interaction is enhanced in presence of hydrogen peroxide and occurs independently of p53/TP53.Interacts with p53/TP53; the interaction is enhanced in response to DNA damage; the interaction is impaired by TSPYL5.Interacts with PTEN; the interaction is direct.Interacts with UBXN6.Interacts with ATXN1 and the strength of interaction is influenced by the length of the poly-Gln region in ATXN1.A weaker interaction seen with mutants having longer poly-Gln regions.Interacts with KIAA1530/UVSSA.Isoform 1 and isoform 2 interact with herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110.Interacts with Epstein-Barr virus EBNA1.EBNA1 shows a 10-fold higher affinity than p53/TP53 and can compete with it for USP7 binding.Binding to ICP0/VMW110 may modulate the substrate specificity or activity of USP7 to stabilize viral proteins.Interacts with MEX3C and antagonizes its ability to degrade mRNA.Interacts with DNMT1 and UHRF1.

Western Blot (WB)

(Western blot detection of HAUSP / USP7 in Hela and K562 cell lysates using HAUSP / USP7 mouse mAb (1:200 diluted), with super ECL.Predicted band size:135KDa.Observed band size:135KDa.)

NCBI and Uniprot Product Information

• NCBI GI #: 1013061510
• NCBI GeneID: 7874
• NCBI Accession #: NP_001308787.1
• NCBI GenBank Nucleotide #: NM_003470
• UniProt Accession #: Q93009; A6NMY8; B7Z815; H0Y3G8
• Molecular Weight: 135
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 7 isoform 4
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 7
• NCBI Official Symbol: USP7
• NCBI Official Synonym Symbols: TEF1; HAUSP
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 7
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 7
• UniProt Gene Name: USP7
• UniProt Synonym Gene Names: HAUSP

NCBI Description

The protein encoded by this gene belongs to the peptidase C19 family, which includes ubiquitinyl hydrolases. This protein deubiquitinates target proteins such as p53 (a tumor suppressor protein) and WASH (essential for endosomal protein recycling), and regulates their activities by counteracting the opposing ubiquitin ligase activity of proteins such as HDM2 and TRIM27, involved in the respective process. Mutations in this gene have been implicated in a neurodevelopmental disorder. [provided by RefSeq, Mar 2016]

Uniprot Description

Hydrolase that deubiquitinates target proteins such as FOXO4, p53/TP53, MDM2, ERCC6, DNMT1, UHRF1, PTEN and DAXX (PubMed:11923872, PubMed:15053880, PubMed:16964248, PubMed:18716620, PubMed:25283148). Together with DAXX, prevents MDM2 self-ubiquitination and enhances the E3 ligase activity of MDM2 towards p53/TP53, thereby promoting p53/TP53 ubiquitination and proteasomal degradation (PubMed:15053880, PubMed:16845383, PubMed:18566590, PubMed:20153724). Deubiquitinates p53/TP53, preventing degradation of p53/TP53, and enhances p53/TP53-dependent transcription regulation, cell growth repression and apoptosis (PubMed:25283148). Deubiquitinates p53/TP53 and MDM2 and strongly stabilizes p53/TP53 even in the presence of excess MDM2, and also induces p53/TP53-dependent cell growth repression and apoptosis (PubMed:11923872). Deubiquitination of FOXO4 in presence of hydrogen peroxide is not dependent on p53/TP53 and inhibits FOXO4-induced transcriptional activity (PubMed:16964248). In association with DAXX, is involved in the deubiquitination and translocation of PTEN from the nucleus to the cytoplasm, both processes that are counteracted by PML (PubMed:18716620). Involved in cell proliferation during early embryonic development. Involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage: recruited to DNA damage sites following interaction with KIAA1530/UVSSA and promotes deubiquitination of ERCC6, preventing UV-induced degradation of ERCC6 (PubMed:22466611, PubMed:22466612). Involved in maintenance of DNA methylation via its interaction with UHRF1 and DNMT1: acts by mediating deubiquitination of UHRF1 and DNMT1, preventing their degradation and promoting DNA methylation by DNMT1 (PubMed:21745816, PubMed:22411829). Acts as a chromatin regulator via its association with the Polycomb group (PcG) multiprotein PRC1-like complex; may act by deubiquitinating components of the PRC1-like complex (PubMed:20601937). Able to mediate deubiquitination of histone H2B; it is however unsure whether this activity takes place in vivo (PubMed:20601937). Exhibits a preference towards 'Lys-48'-linked ubiquitin chains (PubMed:22689415). Increases regulatory T-cells (Treg) suppressive capacity by deubiquitinating and stabilizing the transcription factor FOXP3 which is crucial for Treg cell function (PubMed:23973222).