Rabbit anti-Mouse, Rat DARPP32 Monoclonal Antibody | anti-PPP1R1B antibody

DARPP32 (Dopamine and cAMP-regulated Phosphoprotein, Protein phosphatase 1 regulatory subunit 1B, PP1R1B)

Cat. Num. AAA601945

• Gene Names: PPP1R1B; DARPP32; DARPP-32
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Supernatant; Supernatant
• Synonyms: DARPP32; Monoclonal Antibody; DARPP32 (Dopamine and cAMP-regulated Phosphoprotein; Protein phosphatase 1 regulatory subunit 1B; PP1R1B); Anti -DARPP32 (Dopamine and cAMP-regulated Phosphoprotein; anti-PPP1R1B antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 7H17
• Specificity: Reccognizes endogenous levels of total DARPP-32 protein. Species Crossreactivity
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-PPP1R1B antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence.; Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50; Immunohistochemistry (Paraffin): 1:100 using citrate/TBST for antigen retrieval.; Immunofluorescence (Frozen): 1:400
• Immunogen: Synthetic peptide corresponding to residues surrounding Glu160 of human DARPP-32 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from rat, mouse brain, wild-type mouse striatum and DARPP-32 knock-out mouse striatum, using MBS601945.)

Western Blot (WB)

(Western Blot analysis of extracts from rat and mouse brain using MBS601945.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse brain using MBS601945.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat retina using MBS601945.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of normal rat cerebellum, retina, and ciliary epithelium (as indicated) using MBS601945 (green) and Neurofilament-H mouse mAb (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)

Related Product Information for anti-PPP1R1B antibody

DARPP-32 (dopamine and cyclic AMP-regulated phosphoprotein, relative molecular mass 32,000) is a cytosolic protein highly enriched in medium-sized spiny neurons of the neostriatum (1). It is a bifunctional signaling molecule that controls serine/threonine kinase and serine/threonine phosphatase activity (2). Dopamine stimulates phosphorylation of DARPP-32 through D1 receptors and activation of PKA. PKA phosphorylation of DARPP-32 at threonine 34 converts it into an inhibitor of protein phosphatase 1 (1). DARPP-32 is converted into an inhibitor of PKA when phosphorylated at threonine 75 by cyclin-dependent kinase 5 (CDK5) (2). Mice containing a targeted deletion of the DARPP-32 gene exhibit an altered biochemical, electrophysiological and behavioral phenotype (3).

Product Categories/Family for anti-PPP1R1B antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 21717548
• NCBI GeneID: 84152
• UniProt Accession #: Q9UD71; Q547V9; Q547W0; Q9H7G1
• Molecular Weight: 22,963 Da
• NCBI Official Full Name: dopamine and cAMP-regulated neuronal phosphoprotein
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory (inhibitor) subunit 1B
• NCBI Official Symbol: PPP1R1B
• NCBI Official Synonym Symbols: DARPP32; DARPP-32
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 1B; dopamine and cAMP-regulated neuronal phosphoprotein 32
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 1B
• Protein Family: Protein phosphatase 1 regulatory
• UniProt Gene Name: PPP1R1B
• UniProt Synonym Gene Names: DARPP32
• UniProt Entry Name: PPR1B_HUMAN

NCBI Description

This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

DARPP-32: a member of the protein phosphatase inhibitor 1 family. A dopamine- and cyclic AMP-regulated neuronal phosphoprotein. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions. Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32; phosphorylated DARPP32 is a potent protein phosphatase-1 inhibitor. NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32. Two alternatively-spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: cell soma; cytoplasm; cytosol; nucleus

Molecular Function: protein kinase inhibitor activity; protein phosphatase type 1 regulator activity; D4 dopamine receptor binding; protein phosphatase inhibitor activity; type 1 serine/threonine specific protein phosphatase inhibitor activity; D1 dopamine receptor binding; D3 dopamine receptor binding; D2 dopamine receptor binding; D5 dopamine receptor binding

Biological Process: negative regulation of female receptivity; transcription, DNA-dependent; regulation of catalytic activity; response to amphetamine; negative regulation of catalytic activity; visual learning; negative regulation of protein kinase activity; signal transduction