Rabbit anti-Human PPP1R14A Polyclonal Antibody | anti-PPP1R14A antibody

PPP1R14A (Protein Phosphatase 1 Regulatory Subunit 14A, 17kD PKC-potentiated Inhibitory Protein of PP1, Protein Kinase C-potentiated Inhibitor Protein of 17kD, CPI-17, CPI17, PPP1INL) (FITC)

Cat. Num. AAA6390387

• Gene Names: PPP1R14A; CPI17; CPI-17; PPP1INL
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PPP1R14A; Polyclonal Antibody; PPP1R14A (Protein Phosphatase 1 Regulatory Subunit 14A; 17kD PKC-potentiated Inhibitory Protein of PP1; Protein Kinase C-potentiated Inhibitor Protein of 17kD; CPI-17; CPI17; PPP1INL) (FITC); anti-PPP1R14A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PPP1R14A.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PPP1R14A antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PPP1R14A, aa1-147 (NP_150281.1).
• Immunogen Sequence: MAAQRLGKRVLSKLQSPSRARGPGGSPGGLQKRHARVTVKYDRRELQRRLDVEKWIDGRLEELYRGMEADMPDEINIDELLELESEEERSRKIQGLLKSCGKPVEDFIQELLAKLQGLHRQPGLRQPSPSHDGSLSPLQDRARTAHP
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of PPP1R14A expression in transfected 293T cell line by PPP1R14A polyclonal antibody. Lane 1: PPP1R14A transfected lysate (16.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-PPP1R14A antibody

PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase (see MIM 603768). Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2+) concentration.

Product Categories/Family for anti-PPP1R14A antibody

Antibodies; Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 15147250
• NCBI GeneID: 94274
• NCBI Accession #: NP_150281.1
• NCBI GenBank Nucleotide #: NM_033256.1
• UniProt Accession #: Q96A00; Q7Z4X7; Q96S54
• Molecular Weight: 16,693 Da
• NCBI Official Full Name: protein phosphatase 1 regulatory subunit 14A isoform 1
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory (inhibitor) subunit 14A
• NCBI Official Symbol: PPP1R14A
• NCBI Official Synonym Symbols: CPI17; CPI-17; PPP1INL
• NCBI Protein Information: protein phosphatase 1 regulatory subunit 14A; 17-KDa protein; PKC-potentiated inhibitory protein of PP1; 17 kDa PKC-potentiated inhibitory protein of PP1; 17-kDa PKC-potentiated inhibitory protein of PP1; protein kinase C-potentiated inhibitor protein of
• UniProt Protein Name: Protein phosphatase 1 regulatory subunit 14A
• UniProt Gene Name: PPP1R14A
• UniProt Synonym Gene Names: CPI17; PPP1INL; CPI-17
• UniProt Entry Name: PP14A_HUMAN

NCBI Description

The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

PPP1R14A: a phosphorylation-dependent inhibitory subunit of protein phosphatase 1 (PP-1A). Phosphorylation by PKC regulates its binding to PP-1A. Acts co-operatively to inhibit myosin light chain phosphatase (MLCP) activity. Inhibition leads to increased myosin phosphorylation and enhances smooth muscle contraction in the absence of increased intracellular Ca(2 ) concentration. Involved in the regulation of cell morphology, re-organisation of microfilaments, and cell spreading. Also expressed in human platelets and in brain where it may play a role in cerebellar long-term synaptic depression. Phosphorylation by ILK or Rho kinase enhances its ability to inhibit PP-1A.

Protein type: Protein phosphatase, regulatory subunit; Inhibitor

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm

Molecular Function: protein phosphatase inhibitor activity

Biological Process: regulation of phosphorylation; negative regulation of catalytic activity