Rabbit Map2k4 Polyclonal Antibody | anti-Map2k4 antibody

Map2k4, CT (Map2k4, Jnkk1, Mek4, Mkk4, Prkmk4, Sek1, Serk1, Skk1, Dual specificity mitogen-activated protein kinase kinase 4, C-JUN N-terminal kinase kinase 1, JNK-activating kinase 1, MAPK/ERK kinase 4, SAPK/ERK kinase 1) (AP)

Cat. Num. AAA6318484

• Gene Names: Map2k4; MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
• Reactivity: Human, Mouse, Zebrafish
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Map2k4; Polyclonal Antibody; CT (Map2k4; Jnkk1; Mek4; Mkk4; Prkmk4; Sek1; Serk1; Skk1; Dual specificity mitogen-activated protein kinase kinase 4; C-JUN N-terminal kinase kinase 1; JNK-activating kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1) (AP); SAPK/ERK kinase 1; anti-Map2k4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Zebrafish
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-Map2k4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Map2k4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 258-287 amino acids from the C-terminal region of Mouse Map2k4.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Map2k4 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Map2k4 antibody detected the Map2k4 protein (arrow).)

Western Blot (WB)

(Mouse Map2k4 Antibody (C-term) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the Map2k4 antibody detected the Map2k4 protein (arrow).)

Western Blot (WB)

(Mouse Map2k4 Antibody (C-term) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the Mouse Map2k4 antibody detected the Mouse Map2k4 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Map2k4 Antibody (C-term) (AP14920b)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map2k4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Map2k4 antibody

Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).

Product Categories/Family for anti-Map2k4 antibody

Antibodies; Protein Kinases

References

Finegan, K.G., et al. Cancer Res. 70(14):5797-5806(2010). Ahn, Y.H., et al. J. Biol. Chem. 284(43):29399-29404(2009). Bogani, D., et al. PLoS Biol. 7 (9), E1000196 (2009): Liu, W., et al. Circ. Res. 104(7):905-914(2009). Bulat, N., et al. J. Lipid Res. 50(1):81-89(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 22095023
• NCBI GeneID: 26398
• NCBI Accession #: NP_033183.1
• NCBI GenBank Nucleotide #: NM_009157.4
• UniProt Accession #: P47809
• Molecular Weight: 44,114 Da
• NCBI Official Full Name: dual specificity mitogen-activated protein kinase kinase 4
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 4
• NCBI Official Symbol: Map2k4
• NCBI Official Synonym Symbols: MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 4; MEK 4; JNKK 1; MAPKK 4; JNK kinase 1; MAPK/ERK kinase 4; SAPK/ERK kinase 1; SAPK/Erk/kinase 1; MAP kinase kinase 4; JNK-activating kinase 1; C-JUN N-terminal kinase kinase 1; mitogen activated pr
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 4
• Protein Family: Dual specificity mitogen-activated protein kinase kinase
• UniProt Gene Name: Map2k4
• UniProt Synonym Gene Names: Jnkk1; Prkmk4; Sek1; Serk1
• UniProt Entry Name: MP2K4_MOUSE

Uniprot Description

MKK4: dual specificity kinase of the STE7 family that phosphorylates and activates JNK1 and -2 as well as p38 but not ERK1 or -2. Mediates cellular responses to various cellular stresses and inflammatory cytokines. Phosphorylation by Akt inhibits MKK4 and suppresses stress-activated signal transduction.

Protein type: Protein kinase, dual-specificity (non-receptor); Kinase, protein; Protein kinase, STE; EC 2.7.12.2; STE group; STE7 family

Cellular Component: dendrite cytoplasm; axon; cytoplasm; perikaryon; intracellular; cytosol; nucleus

Molecular Function: transferase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; mitogen-activated protein kinase kinase kinase binding; JUN kinase kinase activity; ATP binding; protein kinase activity

Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of neuron apoptosis; MAPKKK cascade; JNK cascade; positive regulation of protein amino acid phosphorylation; protein amino acid phosphorylation; phosphorylation; positive regulation of DNA replication; activation of JNK activity; positive regulation of nitric-oxide synthase biosynthetic process