Mouse anti-Human PPM1G Monoclonal Antibody | anti-PPM1G antibody

PPM1G, CT (Protein Phosphatase 1G, Protein Phosphatase 1C, Protein Phosphatase 2C Isoform gamma, PP2C-gamma, Protein Phosphatase Magnesium-dependent 1 gamma, PPM1C)

Cat. Num. AAA645156

• Gene Names: PPM1G; PP2CG; PPP2CG; PP2CGAMMA
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PPM1G; Monoclonal Antibody; CT (Protein Phosphatase 1G; Protein Phosphatase 1C; Protein Phosphatase 2C Isoform gamma; PP2C-gamma; Protein Phosphatase Magnesium-dependent 1 gamma; PPM1C); Anti -PPM1G; anti-PPM1G antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: K1G6
• Specificity: Recognizes human PPM1G.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-PPM1G antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1:1000-1:2000; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 5ug/ml
• Immunogen: Recombinant corresponding to human PPM1G aa317-546, expressed in E. coli.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with PPM1G antibody at 5ug/ml followed by biotinylated mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Heart (formalin-fixed, paraffin-embedded) stained with PPM1G antibody at 5ug/ml followed by biotinylated mouse IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Testing Data

(Cell lysates of 293T (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1G (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Related Product Information for anti-PPM1G antibody

PPP1G1 binds magnesium or manganese ions and is widely expressed, being most abundant in testis, skeletal muscle and heart. PPP1G1 is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. PPP1G1 is responsible for the dephosphorylation of ore-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role for this phosphatase in regulating cell cycle progression.

Product Categories/Family for anti-PPM1G antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 29826282
• NCBI GeneID: 5496
• NCBI Accession #: NP_817092.1
• NCBI GenBank Nucleotide #: NM_177983.2
• UniProt Accession #: O15355
• Molecular Weight: 59,272 Da
• NCBI Official Full Name: protein phosphatase 1G
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1G
• NCBI Official Symbol: PPM1G
• NCBI Official Synonym Symbols: PP2CG; PPP2CG; PP2CGAMMA
• NCBI Protein Information: protein phosphatase 1G; PP2C-gamma; PP2C, gamma; protein phosphatase 1C; protein phosphatase 2C gamma isoform; protein phosphatase 2C isoform gamma; protein phosphatase magnesium-dependent 1 gamma; protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
• UniProt Protein Name: Protein phosphatase 1G
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1G
• UniProt Synonym Gene Names: PPM1C; PP2C-gamma
• UniProt Entry Name: PPM1G_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]

Uniprot Description

PPM1G: is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. [provided by RefSeq, Apr 2010]

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis; Spliceosome

Chromosomal Location of Human Ortholog: 2p23.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; protein serine/threonine phosphatase activity

Biological Process: cell cycle arrest; protein amino acid dephosphorylation