Bovine Cadherin, Neuronal ELISA Kit | CDHN elisa kit

Bovine Cadherin, Neuronal ELISA Kit

Cat. Num. AAA741142

• Gene Names: CDH2; CDHN; NCAD; CD325; CDw325
• Reactivity: Bovine
• Synonyms: Cadherin; Neuronal; Bovine Cadherin; Neuronal ELISA Kit; CDHN elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This CDHN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine CDHN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDHN elisa kit

Principle of the assay: CDHN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CDHN antibody and an CDHN-HRP conjugate. The assay sample and buffer are incubated together with CDHN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDHN concentration since CDHN from samples and CDHN-HRP conjugate compete for the anti-CDHN antibody binding site. Since the number of sites is limited, as more sites are occupied by CDHN from the sample, fewer sites are left to bind CDHN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDHN concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CDHN elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119621646
• NCBI GeneID: 1000
• Molecular Weight: 99,809 Da
• NCBI Official Full Name: cadherin 2, type 1, N-cadherin (neuronal), isoform CRA_b
• NCBI Official Synonym Full Names: cadherin 2, type 1, N-cadherin (neuronal)
• NCBI Official Symbol: CDH2
• NCBI Official Synonym Symbols: CDHN; NCAD; CD325; CDw325
• NCBI Protein Information: cadherin-2; N-cadherin 1; neural cadherin; neural-cadherin; cadherin 2, N-cadherin (neuronal); calcium-dependent adhesion protein, neuronal
• UniProt Protein Name: Cadherin-2
• UniProt Gene Name: CDH2
• UniProt Synonym Gene Names: CDHN; NCAD; N-cadherin
• UniProt Entry Name: CADH2_HUMAN

NCBI Description

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq, Jul 2008]

Uniprot Description

CDH2: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. Interacts with CDCP1. Identified in a complex containing FGFR4, NCAM1, CDH2, PLCG1, FRS2, SRC, SHC1, GAP43 and CTTN. Interacts with PCDH8; this complex may also include TAOK2. The interaction with PCDH8 may lead to internalization through TAOK2/p38 MAPK pathway.

Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 18q11.2

Cellular Component: focal adhesion; cell-cell adherens junction; lamellipodium; apical plasma membrane; plasma membrane; integral to membrane; synapse; intercellular junction; catenin complex; fascia adherens

Molecular Function: protein binding; gamma-catenin binding; beta-catenin binding; calcium ion binding; protein phosphatase binding; alpha-catenin binding

Biological Process: heterophilic cell adhesion; intercellular junction assembly and maintenance; muscle cell differentiation; cell migration; glial cell differentiation; positive regulation of MAPKKK cascade; calcium-dependent cell-cell adhesion; positive regulation of muscle cell differentiation; striated muscle cell differentiation; blood vessel morphogenesis; cell adhesion; homophilic cell adhesion