Sheep Peroxisome proliferators activator gamma ELISA Kit | PPAR-gamma elisa kit

Sheep Peroxisome proliferators activator gamma ELISA Kit

Cat. Num. AAA739113

• Gene Names: PPARG; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma
• Reactivity: Sheep
• Synonyms: Peroxisome proliferators activator gamma; Sheep Peroxisome proliferators activator gamma ELISA Kit; Sheep Peroxisome proliferators activator g ELISA Kit; PPAR-gamma elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This PPAR-gamma ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep PPAR-gamma. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PPAR-gamma elisa kit

Principle of the Assay: PPAR-gamma ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PPAR-gamma antibody and an PPAR-gamma-HRP conjugate. The assay sample and buffer are incubated together with PPAR-gamma-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PPAR-gamma concentration since PPAR-gamma from samples and PPAR-gamma-HRP conjugate compete for the anti-PPAR-gamma antibody binding site. Since the number of sites is limited, as more sites are occupied by PPAR-gamma from the sample, fewer sites are left to bind PPAR-gamma-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPAR-gamma concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13432234
• NCBI GeneID: 5468
• Molecular Weight: 21,580 Da
• NCBI Official Full Name: Peroxisome proliferator-activated receptor gamma
• NCBI Official Synonym Full Names: peroxisome proliferator-activated receptor gamma
• NCBI Official Symbol: PPARG
• NCBI Official Synonym Symbols: GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma
• NCBI Protein Information: peroxisome proliferator-activated receptor gamma; PPAR-gamma; nuclear receptor subfamily 1 group C member 3; peroxisome proliferator-activated nuclear receptor gamma variant 1
• UniProt Protein Name: Peroxisome proliferator-activated receptor gamma
• UniProt Gene Name: PPARG
• UniProt Synonym Gene Names: NR1C3; PPAR-gamma
• UniProt Entry Name: PPARG_HUMAN

NCBI Description

This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

PPAR-gamma: a transcription factor, member of the nuclear hormone receptor superfamily. Receptor for hypolipidemic drugs and fatty acids. Preferentially expressed in adipocytes as well as in vascular smooth muscle cells and macrophage. Regulator of adipogenesis and lipid metabolism, modulates insulin sensitivity, cell proliferation and inflammation. Phosphorylated and inhibited by MAP kinase. Heterodimerizes with the retinoid X receptor. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Two splice-variant isoforms have been described.

Protein type: Nuclear receptor; DNA-binding

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; cytosol; nucleus

Molecular Function: ligand-dependent nuclear receptor activity; transcription activator binding; zinc ion binding; drug binding; alpha-actinin binding; protein phosphatase binding; arachidonic acid binding; retinoid X receptor binding; protein binding; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; DNA binding; prostaglandin receptor activity; sequence-specific DNA binding; steroid hormone receptor activity; estrogen receptor binding; chromatin binding; transcription factor activity

Biological Process: negative regulation of collagen biosynthetic process; positive regulation of transcription, DNA-dependent; heart development; cell maturation; rhythmic process; lipid homeostasis; glucose homeostasis; response to lipid; response to vitamin A; response to caffeine; positive regulation of oligodendrocyte differentiation; placenta development; long-chain fatty acid transport; organ regeneration; cell fate commitment; monocyte differentiation; negative regulation of acute inflammatory response; regulation of circadian rhythm; response to starvation; negative regulation of telomerase activity; cellular response to insulin stimulus; response to mechanical stimulus; response to estrogen stimulus; lipoprotein transport; brown fat cell differentiation; positive regulation of fat cell differentiation; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; fatty acid oxidation; positive regulation of transcription factor activity; positive regulation of phagocytosis, engulfment; negative regulation of transcription, DNA-dependent; negative regulation of smooth muscle cell proliferation; low-density lipoprotein receptor biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; signal transduction; epithelial cell differentiation; regulation of blood pressure; response to nutrient; caspase activation; transcription initiation from RNA polymerase II promoter; response to retinoic acid; G-protein coupled receptor protein signaling pathway; response to low density lipoprotein stimulus; positive regulation of fatty acid oxidation; white fat cell differentiation; innate immune response; gene expression; lipid metabolic process; negative regulation of cell growth; response to cold

Disease: Obesity; Carotid Intimal Medial Thickness 1; Lipodystrophy, Familial Partial, Type 3; Diabetes Mellitus, Noninsulin-dependent