Human Iron Responsive Element Binding Protein 2 ELISA Kit | IREB2 elisa kit

Human IREB2 (Iron Responsive Element Binding Protein 2) ELISA Kit

Cat. Num. AAA2533421

• Gene Names: IREB2; ACO3; IRP2; IRP2AD
• Reactivity: Human
• Synonyms: Iron Responsive Element Binding Protein 2; Human IREB2 (Iron Responsive Element Binding Protein 2) ELISA Kit; IREB2 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinantHumanIREB2. No significant cross-reactivity or interference between HumanIREB2 and analogues was observed.
• Sequence Length: 963

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL
• Sensitivity: Min: 0.188ng/mL; Max: 20ng/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IREB2 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of HumanIREB2concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IREB2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IREB2and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IREB2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IREB2.You can calculate the concentration of IREB2in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 41352693
• NCBI GeneID: 3658
• NCBI Accession #: NP_004127.1
• NCBI GenBank Nucleotide #: NM_004136.2
• Molecular Weight: 37,487 Da
• NCBI Official Full Name: iron-responsive element-binding protein 2
• NCBI Official Synonym Full Names: iron responsive element binding protein 2
• NCBI Official Symbol: IREB2
• NCBI Official Synonym Symbols: ACO3; IRP2; IRP2AD
• NCBI Protein Information: iron-responsive element-binding protein 2
• UniProt Protein Name: Iron-responsive element-binding protein 2
• Protein Family: Iron-responsive element-binding protein
• UniProt Gene Name: IREB2
• UniProt Synonym Gene Names: IRE-BP 2; IRP2
• UniProt Entry Name: IREB2_HUMAN

Uniprot Description

IREB2: RNA-binding protein that binds to iron-responsive elements (IRES), which are stem-loop structures found in the 5'- UTR of ferritin, and delta aminolevulinic acid synthase mRNAs, and in the 3'-UTR of transferrin receptor mRNA. Binding to the IRE element in ferritin results in the repression of its mRNA translation. Binding of the protein to the transferrin receptor mRNA inhibits the degradation of this otherwise rapidly degraded mRNA. Belongs to the aconitase/IPM isomerase family.

Protein type: RNA-binding; Translation

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: cytoplasm

Molecular Function: 4 iron, 4 sulfur cluster binding; iron-responsive element binding; metal ion binding; protein binding; RNA binding; translation repressor activity

Biological Process: cellular iron ion homeostasis; iron ion transport; metabolic process; negative regulation of translation