Rabbit anti-Human, Mouse PP5 Polyclonal Antibody | anti-PP5 antibody

PP5 (protein phosphatase 5, catalytic subunit, FLJ36922, PP5, PPP5, PPT, PP-T, Protein phosphatase T, Serine/threonine-protein Phosphatase 5) (FITC)

Cat. Num. AAA6495601

• Gene Names: PPP5C; PP5; PPT; PPP5
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Purified
• Synonyms: PP5; Polyclonal Antibody; PP5 (protein phosphatase 5; catalytic subunit; FLJ36922; PPP5; PPT; PP-T; Protein phosphatase T; Serine/threonine-protein Phosphatase 5) (FITC); anti-PP5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human protein phosphatase 5. Species Crossreactivity: mouse.
• Purity/Purification: Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-PP5 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide derived from the human protein phosphatase 5. Cellular Localization: Nucleus. Cytoplasm. Epitope: C-terminus.
• Conjugate: FITC
• Positive Control: Jurkat Cell Lysate, A549 and MCF-7 cell lysates.
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of PP5 Polyclonal Antibody: Human Testis PP5 staining of round spermatids in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.)

Western Blot (WB)

(Western Blot Analysis: Jurkat lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-5 (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-5 (~58kD).)

Western Blot (WB)

(Western Blot Analysis: Representative image from a previous lot. Jurkat lysate (Lane1), RAW 264 lysate (Lane2), HEK293 lysate (Lane 3), NIH/3T3 lysate (Lane 4), C2C12 lysate (Lane 5), Huvec lysate (Lane 6), A431 lysate (Lane 7), HepG2 lysate (Lane 8), Hela lysate (Lane 9) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-5 (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-5 (~58kD).)

Product Categories/Family for anti-PP5 antibody

Antibodies; Enzymes; Phosphatase

References

1. Chinkers, M., et al. (2001). Trends Endocrinol. Metab. 12, 28-32. 2. Zuo, Z., et al. (1999). Biochemistry. 38, 8849-8857. 3. Chen, MX, et al. (1994). MBO J. 13, 4278-4290.

NCBI and Uniprot Product Information

• NCBI GI #: 5453958
• NCBI GeneID: 5536
• NCBI Accession #: NP_006238.1
• NCBI GenBank Nucleotide #: NM_006247.2
• UniProt Accession #: P53041; Q16722; Q53XV2
• Molecular Weight: 56,879 Da
• NCBI Official Full Name: serine/threonine-protein phosphatase 5 isoform 1
• NCBI Official Synonym Full Names: protein phosphatase 5, catalytic subunit
• NCBI Official Symbol: PPP5C
• NCBI Official Synonym Symbols: PP5; PPT; PPP5
• NCBI Protein Information: serine/threonine-protein phosphatase 5; PP-T; protein phosphatase T
• UniProt Protein Name: Serine/threonine-protein phosphatase 5
• UniProt Gene Name: PPP5C
• UniProt Synonym Gene Names: PPP5; PP5; PP-T; PPT
• UniProt Entry Name: PPP5_HUMAN

NCBI Description

This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]

Uniprot Description

Function: May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.

Catalytic activity: A phosphoprotein + H2O = a protein + phosphate.

Cofactor: Binds 1 iron ion per subunit

By similarity.Binds 1 manganese ion per subunit

By similarity.

Subunit structure: Interacts with CDC16 and CDC27. Ref.8

Subcellular location: Nucleus. Cytoplasm. Note: Predominantly nuclear. But also present in the cytoplasm.

Tissue specificity: Ubiquitous.

Sequence similarities: Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.Contains 3 TPR repeats.