Rabbit MAP1LC3A/B Polyclonal Antibody | anti-MAP1LC3A/B antibody

RABBIT ANTI HUMAN MAP1LC3A/B (N-TERMINAL)

Cat. Num. AAA224212

• Gene Names: MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
• Applications: Immunofluorescence, Western Blot
• Synonyms: MAP1LC3A/B; Polyclonal Antibody; RABBIT ANTI HUMAN MAP1LC3A/B (N-TERMINAL); anti-MAP1LC3A/B antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 125

• Applicable Applications for anti-MAP1LC3A/B antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 14-15kDa corresponding to LC3-II, and a band of approximately 17kDa corresponding to LC3-I, in HeLa cell lysates. ; Western Blotting: Minimum Dilution: 1/100; Maximum Dilution: 1/250
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide sequence PSDRPFKQRRSFADC from the N-Terminal region of LC3A (NP_115903.1; NP_852610.1).; Buffer Solution
• Antiserum Preparation: Antiserum to human LC3A was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(HeLa lysate probed with Rabbit anti Human MAP1LC3A/B (N-terminal) (MBS224211) followed by Sheep anti Rabbit IgG:HRP (MBS235194))

Testing Data #2

(Western blotting analysis of LC3 expression on Atg5 knockout and wild type cells using Rabbit anti Human MAP1LC3A/B (MBS224211). Image courtesy of Dr. R. Kapito, Stanford University)

Related Product Information for anti-MAP1LC3A/B antibody

Rabbit anti Human MAP1LC3A/B (N-Terminal) antibody specifically recognizes an epitope within the N-Terminal (NT) region of both MAP1LC3A (Microtubule-associated proteins 1A/1B light chain 3A/LC3A) and MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B/LC3B), ubiquitin-like proteins and members of the MAP1LC3 family, which are widely used as reliable markers for the monitoring of autophagy. LC3-I is the cytosolic form of LC3, which is converted into the active, membrane-bound form LC3-II, during the autophagy process. Tracking the level of conversion of LC3-I to LC3-II provides an indicator of autophagic activity, and levels of LC3-II in particular, correlate with the extent of autophagosome formation, due to its association with the autophagosome membrane. Rabbit anti Human MAP1LC3A/B (N-Terminal) antibody recognizes both the LC3-I and LC3-II forms of MAP1LC3A and MAP1LC3B.

NCBI and Uniprot Product Information

• NCBI GI #: 12383056
• NCBI GeneID: 81631
• NCBI Accession #: NP_073729.1
• NCBI GenBank Nucleotide #: NM_022818.4
• Molecular Weight: 14,688 Da
• NCBI Official Full Name: microtubule-associated proteins 1A/1B light chain 3B
• NCBI Official Synonym Full Names: microtubule-associated protein 1 light chain 3 beta
• NCBI Official Symbol: MAP1LC3B
• NCBI Official Synonym Symbols: LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
• NCBI Protein Information: microtubule-associated proteins 1A/1B light chain 3B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; autophagy-related ubiquitin-like modifier LC3 B
• UniProt Protein Name: Microtubule-associated proteins 1A/1B light chain 3B
• UniProt Gene Name: MAP1LC3B
• UniProt Synonym Gene Names: MAP1ALC3; MAP1A/MAP1B LC3 B
• UniProt Entry Name: MLP3B_HUMAN

NCBI Description

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]

Uniprot Description

LC3B: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Recruits proteins such as SQSTM1 and NBR1 to the autophagosome.

Protein type: Vesicle; Microtubule-binding; Ubiquitin-like modifier; Autophagy

Chromosomal Location of Human Ortholog: 16q24.2

Cellular Component: microtubule; extrinsic to membrane; mitochondrion; endomembrane system; autophagic vacuole; cytoplasmic vesicle; axoneme; intracellular; cytosol

Molecular Function: protein binding; microtubule binding; GABA receptor binding

Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation