Immunohistochemistry (IHC)
(Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of PP5 Polyclonal Antibody: Human Testis PP5 staining of round spermatids in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.)
Rabbit anti-Human, Mouse PP5 Polyclonal Antibody | anti-PP5 antibody
PP5 (protein phosphatase 5, catalytic subunit, FLJ36922, PP5, PPP5, PPT, PP-T, Protein phosphatase T, Serine/threonine-protein Phosphatase 5) (MaxLight 405)
Applications are based on unconjugated antibody.
Immunohistochemistry (IHC)
(Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of PP5 Polyclonal Antibody: Human Testis PP5 staining of round spermatids in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.)
Immunohistochemistry (IHC)
(Immunohistochemistry(paraffin): Representative images from a previous lot. Optimal Staining of PP5 Polyclonal Antibody: Human Testis PP5 staining of round spermatids in normal human testis, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1:100, using IHC-Select detection with HRP-DAB.)
Western Blot (WB)
(Western Blot Analysis: Jurkat lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-5 (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-5 (~58kD).)
Western Blot (WB)
(Western Blot Analysis: Jurkat lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-5 (1:500 dilution of this lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-5 (~58kD).)
Western Blot (WB)
(Western Blot Analysis: Representative image from a previous lot. Jurkat lysate (Lane1), RAW 264 lysate (Lane2), HEK293 lysate (Lane 3), NIH/3T3 lysate (Lane 4), C2C12 lysate (Lane 5), Huvec lysate (Lane 6), A431 lysate (Lane 7), HepG2 lysate (Lane 8), Hela lysate (Lane 9) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-5 (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-5 (~58kD).)
Western Blot (WB)
(Western Blot Analysis: Representative image from a previous lot. Jurkat lysate (Lane1), RAW 264 lysate (Lane2), HEK293 lysate (Lane 3), NIH/3T3 lysate (Lane 4), C2C12 lysate (Lane 5), Huvec lysate (Lane 6), A431 lysate (Lane 7), HepG2 lysate (Lane 8), Hela lysate (Lane 9) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP-5 (1:500 dilution of a previous lot). Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates protein PP-5 (~58kD).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]
Uniprot Description
Function: May play a role in the regulation of RNA biogenesis and/or mitosis. In vitro, dephosphorylates serine residues of skeletal muscle phosphorylase and histone H1.
Catalytic activity: A phosphoprotein + H2O = a protein + phosphate.
Cofactor: Binds 1 iron ion per subunit
By similarity.Binds 1 manganese ion per subunit
By similarity.
Subunit structure: Interacts with CDC16 and CDC27. Ref.8
Subcellular location: Nucleus. Cytoplasm. Note: Predominantly nuclear. But also present in the cytoplasm.
Tissue specificity: Ubiquitous.
Sequence similarities: Belongs to the PPP phosphatase family. PP-5 (PP-T) subfamily.Contains 3 TPR repeats.
Research Articles on PP5
Similar Products
Product Notes
The PP5 ppp5c (Catalog #AAA6495603) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PP5 (protein phosphatase 5, catalytic subunit, FLJ36922, PP5, PPP5, PPT, PP-T, Protein phosphatase T, Serine/threonine-protein Phosphatase 5) (MaxLight 405) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PP5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:1000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the PP5 ppp5c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
