Mouse anti-Human HSPA1A Monoclonal Antibody | anti-HSPA1A antibody

HSPA1A (HSPA1A, HSPA1, Heat shock 70kD protein 1A/1B, Heat shock 70kD protein 1/2) (PE)

Cat. Num. AAA6121692

• Gene Names: HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: HSPA1A; Monoclonal Antibody; HSPA1A (HSPA1A; HSPA1; Heat shock 70kD protein 1A/1B; Heat shock 70kD protein 1/2) (PE); Heat shock 70kD protein 1/2; anti-HSPA1A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 201CT8.2.3
• Specificity: Recognizes human HSPA1A.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-HSPA1A antibody: FLISA, Immunofluorescence (IF), Western Blot (WB)
• Application Notes: FLISA: 1:1000; IF: 1:10-50; WB: 1:100-2000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 574-600 of human HSPA1A, UniProt Accession #P08107. Species sequence homology: bovine, porcine
• Conjugate: PE
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze R-Phycoerythrin conjugates. Light sensitive.

Western Blot (WB)

(Western blot analysis of in HL-60 cell line lysates (35ug/lane).This demonstrates the HSPA1A antibody detected the HSPA1A protein (arrow).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of with Hela cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-HSPA1A antibody

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Product Categories/Family for anti-HSPA1A antibody

Antibodies; Heat Shock Proteins

References

Wang, Y., et al. Clin. Chem. Lab. Med. 48(11):1657-1663(2010)Eisenberg, D.P., et al. Surgery 148(2):325-334(2010) Rusai, K., et al. Transplant. Proc. 42(6):2309-2311(2010) Ebrahimi, M., et al. Mol. Vis. 16, 1680-1688 (2010) Lanneau, D., et al. ScientificWorldJournal 10, 1543-1552 (2010)

NCBI and Uniprot Product Information

• NCBI GI #: 194248072
• NCBI GeneID: 3303
• NCBI Accession #: NP_005336.3
• NCBI GenBank Nucleotide #: NM_005345.5
• UniProt Accession #: P08107; P19790; Q5JQI4; Q5SP17; Q9UQL9; Q9UQM0; B4E3B6
• Molecular Weight: 70,052 Da
• NCBI Official Full Name: heat shock 70 kDa protein 1A/1B
• NCBI Official Synonym Full Names: heat shock 70kDa protein 1A
• NCBI Official Symbol: HSPA1A
• NCBI Official Synonym Symbols: HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A
• NCBI Protein Information: heat shock 70 kDa protein 1A/1B; HSP70-1/HSP70-2; heat shock 70kD protein 1A; heat shock-induced protein; heat shock 70 kDa protein 1/2; dnaK-type molecular chaperone HSP70-1
• UniProt Protein Name: Heat shock 70 kDa protein 1A/1B
• Protein Family: Heat shock 70 kDa protein
• UniProt Gene Name: HSPA1A
• UniProt Synonym Gene Names: HSPA1; HSP70-1/HSP70-2
• UniProt Entry Name: HSP71_HUMAN

NCBI Description

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

HSP70: a critical chaperone protein that has a high affinity for unfolded polypeptide chains. It binds extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In cooperation with other chaperones, hsp70 stabilizes preexistent proteins against aggregation and mediates the folding of newly translated polypeptides in the cytosol as well as within organelles. Mitochondrial HSP70 is crucial to the import process: mutant forms of HSP70 fail to import precursor proteins. Is anti-apoptotic in sympathetic neurones and mediates this effect primarily by suppressing c-Jun transcriptional signalling. Interacts with tau protein and mediates proper folding of tau. Can promote the degradation of tau protein. Triptolide, a potential therapeutic agent for progression/metastasis of pancreatic cancer, causes pancreatic cancer cell death by induction of apoptosis, an effect mediated by the inhibition of HSP70.

Protein type: Heat shock protein; Motility/polarity/chemotaxis; Chaperone

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: signalosome; focal adhesion; mitochondrion; endoplasmic reticulum; inclusion body; cytosol; ribonucleoprotein complex; centriole; nucleoplasm; perinuclear region of cytoplasm; cytoplasm; nuclear speck; nucleus; vesicle; ubiquitin ligase complex

Molecular Function: viral receptor activity; protein binding; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; ubiquitin protein ligase binding; double-stranded RNA binding; ATPase activity; unfolded protein binding; protein N-terminus binding; ATPase activity, coupled; ATP binding

Biological Process: negative regulation of cell proliferation; entry of virus into host cell; protein stabilization; positive regulation of erythrocyte differentiation; mRNA catabolic process; gene expression; negative regulation of protein ubiquitination; protein refolding; negative regulation of cell growth; response to unfolded protein; negative regulation of apoptosis