Pig complement component 3 ELISA Kit | C3 elisa kit

Pig Complement 3, C3 ELISA Kit

Cat. Num. AAA702654

• Reactivity: Pig
• Synonyms: complement component 3; Pig Complement 3; C3 ELISA Kit; AHUS5; ARMD9; ASP; CPAMD1; acylation-stimulating protein cleavage product; complement component C3; C3 elisa kit

• Reactivity: Pig
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pig C3. No significant cross-reactivity or interference between Pig C3 and analogues was observed.
• Sequence Length: 1661

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 30 ug/ml-1200 ug/ml
• Sensitivity: 15 ug/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Complement
• Protein Biological Process 3: Complement alternate pathway
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C3 elisa kit

Introduction: Expiration date six months from the date of manufacture FOR RESEARCH USE ONLY.. 1 INTRODUCTION C3 comprises about 70% of the total protein in the complement system and is central to activation of both the classical and alternate pathways. Increased levels are found in numerous inflammatory states as an acute phase response. CH50 (total complement hemolytic activity), C3 and/or C4 may be decreased in cases of systemic lupus erythematosus, especially in cases with lupus nephritis, acute and chronic hypocomplementemic nephritis, subacute bacterial endocarditis, DIC, and partial lipodystrophy (with associated nephritis-like activity in serum.) In cases of disseminated intravascular coagulation, plasmin attacks C3 directly, and C3 levels have been found low in the hemolytic uremic syndrome form of disseminated intravascular coagulation (DIC). Cases of hereditary C3 deficiency, while rare, have been reported and are characterized clinically by recurrent infections (eg, pneumonia, meningitis, paronychia, impetigo). Pathogenic bacteria causing infections in these cases have included both gram-positive and gram-negative organisms. C3 levels have also been found deficient in cases of uremia, chronic liver diseases, anorexia nervosa, and celiac disease.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to C3. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated monoclonal antibody preparation specific for C3 and incubated. Then substrate solution A and B are added to each well. Only those wells that contain C3, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of C3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 99028969
• NCBI GeneID: 280677
• NCBI Accession #: NP_001035559.2
• NCBI GenBank Nucleotide #: NM_001040469.2
• UniProt Accession #: Q2UVX4; Q2KIZ4
• Molecular Weight: 187,253 Da
• NCBI Official Full Name: complement C3 preproprotein
• NCBI Official Synonym Full Names: complement component 3
• NCBI Official Symbol: C3
• NCBI Protein Information: complement C3
• UniProt Protein Name: Complement C3
• Protein Family: Complement C3
• UniProt Gene Name: C3
• UniProt Synonym Gene Names: ASP
• UniProt Entry Name: CO3_BOVIN

Uniprot Description

Function: C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates